一种源自登革热的单克隆抗体在体外增强寨卡病毒感染方面的效用
Utility of a Dengue-Derived Monoclonal Antibody to Enhance Zika Infection In Vitro.
作者信息
Charles Anu Susan, Christofferson Rebecca C
机构信息
Pathobiological Sciences, Louisiana State University, Baton Rouge, Louisiana, USA.
出版信息
PLoS Curr. 2016 Jul 5;8:ecurrents.outbreaks.4ab8bc87c945eb41cd8a49e127082620. doi: 10.1371/currents.outbreaks.4ab8bc87c945eb41cd8a49e127082620.
INTRODUCTION
Zika virus (ZIKV) has emerged in dengue (DENV) endemic areas, where these two related flaviviruses continue to co-circulate. DENV is a complex of four serotypes and infections can progress to severe disease. It is thought that this is mediated by antibody dependent enhancement (ADE) whereby antibodies from a primary DENV infection are incapable of neutralizing heterologous DENV infections with another serotype. ADE has been demonstrated among other members of the Flavivirus group.
METHODS
We utilize an in vitro ADE assay developed for DENV to determine whether ZIKV is enhanced by a commonly available DENV serotype 2-derived monoclonal antibody (4G2).
RESULTS
We show that ZIKV infection in vitro is enhanced in the presence of the 4G2 mAb.
DISCUSSION
Our results demonstrate that ADE between ZIKV and DENV is possible and that the 4G2 antibody is a useful tool for the effects of pre-existing anti-DENV antibodies during ZIKV infections.
引言
寨卡病毒（ZIKV）已在登革热（DENV）流行地区出现，这两种相关的黄病毒在这些地区持续共同传播。登革热病毒是一个由四种血清型组成的复合体，感染可能会发展为严重疾病。据认为，这是由抗体依赖性增强（ADE）介导的，即初次感染登革热病毒产生的抗体无法中和感染另一种血清型的异源登革热病毒。ADE在黄病毒科的其他成员中也有发现。
方法
我们利用为登革热病毒开发的体外ADE检测方法，来确定寨卡病毒是否会被一种常用的源自登革热病毒2型的单克隆抗体（4G2）增强。
结果
我们发现，在4G2单克隆抗体存在的情况下，寨卡病毒的体外感染会增强。
讨论
我们的结果表明，寨卡病毒和登革热病毒之间可能存在ADE，并且4G2抗体是研究寨卡病毒感染期间预先存在的抗登革热病毒抗体作用的有用工具。
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