Sinsky R J, Piesman J
Department of Epidemiology, School of Public Health, University of Alabama, Birmingham 35294.
J Clin Microbiol. 1989 Aug;27(8):1723-7. doi: 10.1128/jcm.27.8.1723-1727.1989.
An ear punch biopsy method for the detection and isolation of Borrelia burgdorferi from rodents was developed. The ear punch biopsy proved to be extremely sensitive, detecting spirochetes in 100% (11 of 11) of laboratory hamsters infected by tick bite and 95.8% (23 of 24) of hamsters infected by intraperitoneal inoculation. When cultured at 4 to 6 weeks postinfection, 92 to 100% of the ear punches taken from individual hamsters yielded viable spirochetes. B. burgdorferi was detected in sequential cultures from animals as early as 4 days postinfection and as late as 20 weeks postinfection. A total of 86% (6 of 7) of field-collected white-footed mice (Peromyscus leucopus) which were positive for B. burgdorferi as determined by xenodiagnosis were also positive by the ear punch method. The ear punch biopsy method allows individual rodents to be sampled for B. burgdorferi serially over a long period and thus should prove useful for both field and laboratory experiments.
开发了一种用于从啮齿动物中检测和分离伯氏疏螺旋体的耳打孔活检方法。耳打孔活检被证明极其灵敏，在100%（11只中的11只）被蜱叮咬感染的实验室仓鼠和95.8%（24只中的23只）经腹腔接种感染的仓鼠中检测到螺旋体。在感染后4至6周进行培养时，从个体仓鼠采集的耳组织中有92%至100%产生了活的螺旋体。在感染动物的连续培养物中，最早在感染后4天、最晚在感染后20周检测到伯氏疏螺旋体。通过接种诊断法确定为伯氏疏螺旋体阳性的野外捕获的白足鼠（白足鼠属）中，共有86%（7只中的6只）通过耳打孔法检测也呈阳性。耳打孔活检方法允许在很长一段时间内对单个啮齿动物进行伯氏疏螺旋体的连续采样，因此对于野外和实验室实验都应是有用的。
