Brown Simon H J, Eather Samuel R, Freeman Dilys J, Meyer Barbara J, Mitchell Todd W
School of Biology, University of Wollongong, Wollongong, NSW, Australia.
Illawarra Health and Medical Research Institute, University of Wollongong, Wollongong, NSW, Australia.
PLoS One. 2016 Sep 29;11(9):e0163972. doi: 10.1371/journal.pone.0163972. eCollection 2016.
In preeclampsia, maternal insulin resistance leads to defective expansion of adipocytes, enhanced adipocyte lipolysis, up-regulation of very low density lipoprotein synthesis, maternal hypertriglyceridaemia and the potential for ectopic fat storage. Our aim was to quantitate and compare the total amount and type of lipid in placenta from pregnancies complicated with preeclampsia and healthy pregnancies. Quantitative lipid analysis of lipid extracts from full thickness placental biopsies was carried out by shotgun lipidomics. Placental lipid profiles from pregnancies complicated by preeclampsia (n = 23) were compared to healthy pregnancies (n = 68), and placenta from intrauterine growth restriction pregnancies (n = 10) were used to control for gross differences in placental pathology. Placentae from pregnancies complicated with preeclampsia had higher neutral lipid content than healthy placentae (40% higher triacyglycerol (P = 0.001) and 33% higher cholesteryl ester (P = 0.004)) that was specific to preeclampsia and independent of maternal gestation.
在子痫前期，母体胰岛素抵抗会导致脂肪细胞扩张缺陷、脂肪细胞脂解增强、极低密度脂蛋白合成上调、母体高甘油三酯血症以及异位脂肪储存的可能性。我们的目的是定量并比较子痫前期妊娠和健康妊娠胎盘脂质的总量及类型。通过鸟枪法脂质组学对全层胎盘活检组织的脂质提取物进行定量脂质分析。将子痫前期妊娠（n = 23）的胎盘脂质谱与健康妊娠（n = 68）进行比较，并使用宫内生长受限妊娠（n = 10）的胎盘来控制胎盘病理学的总体差异。子痫前期妊娠的胎盘中性脂质含量高于健康胎盘（甘油三酯高40%（P = 0.001），胆固醇酯高33%（P = 0.004）），这是子痫前期特有的，且与母体孕周无关。
