Stalin Nattan, Srinivasan Pappu
Molecular Biology Laboratory, Department of Bioinformatics, Alagappa University, Karaikudi, 630 003, Tamilnadu, India.
Department of Animal Health and Management, Alagappa University, Karaikudi, 630 003, Tamilnadu, India.
Biologicals. 2016 Nov;44(6):526-533. doi: 10.1016/j.biologicals.2016.08.003. Epub 2016 Sep 30.
Phage therapy is an alternative and eco-friendly biocontrol agent to prevent and control multidrug resistant bacteria in the aquatic system. The aim of this study is to isolate and characterize the Vibrio parahaemolyticus and its potential lytic phage from Penaeus monodon growing-out by rearing in shrimp ponds in Palk Strait, South East coast of India. The conventional phenotypic characteristics and molecular identification was confirmed using 16S rRNA sequence and to determine the antibiotic resistant profiles. The V. parahaemolyticus phage was effective against V. parahaemolyticus through one-step growth experiments, phage survival was determined by long-term storage at various temperatures and pH. Further, transmission electron microscope (TEM) revealed that the lytic phage belongs to the Myoviridae family. The isolated lytic phage (VVP1) was more specific against N1A V. parahaemolyticus and was able to infect N7A V. parahaemolyticus, N3B and N13B Vibrio alginolyticus strains. Evaluation of microcosm studies with P. monodon larvae infected with V. parahaemolyticus showed the survival of larvae in the presence of phage treatment at 2.3 × 10 PFU/mL was enhanced when compared with the control. This study provides the application of phage as a useful strategy to prevent and eliminate or reduce shrimp pathogenic V. parahaemolyticus in the aquaculture system.
噬菌体疗法是一种用于预防和控制水生系统中多重耐药细菌的替代且环保的生物防治剂。本研究的目的是从印度东南海岸帕尔卡海峡虾塘养殖的斑节对虾中分离并鉴定副溶血性弧菌及其潜在的裂解性噬菌体。使用16S rRNA序列确认了传统的表型特征和分子鉴定，并确定了抗生素耐药谱。通过一步生长实验，副溶血性弧菌噬菌体对副溶血性弧菌有效，通过在不同温度和pH下长期保存来确定噬菌体的存活率。此外，透射电子显微镜（TEM）显示该裂解性噬菌体属于肌尾噬菌体科。分离出的裂解性噬菌体（VVP1）对N1A副溶血性弧菌更具特异性，并且能够感染N7A副溶血性弧菌、N3B和N13B溶藻弧菌菌株。对感染副溶血性弧菌的斑节对虾幼体进行的微观研究评估表明，与对照组相比，在2.3×10 PFU/mL的噬菌体处理下，幼体的存活率有所提高。本研究提供了噬菌体作为一种有用策略在水产养殖系统中预防和消除或减少虾致病性副溶血性弧菌的应用。
