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新型副溶血性弧菌噬菌体vB_VpaM_PVP-XSN的分离与鉴定

Isolation and characterization of the novel Vibrio parahaemolyticus phage vB_VpaM_PVP-XSN.

作者信息

Qi Tianpeng, Lyu Sunjian, Xia Meiwen, Lou Bao, Liu Li, Xu Haisheng

机构信息

College of Animal Sciences, Zhejiang University, 866 Yuhangtang Road, Hangzhou, Zhejiang, 310021, China.

State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro- Products, Institute of Hydrobiology, Zhejiang Academy of Agricultural Sciences, 198, Shiqiao Road, Hangzhou, Zhejiang, 310021, China.

出版信息

Arch Virol. 2025 Feb 11;170(3):53. doi: 10.1007/s00705-025-06237-1.

DOI:10.1007/s00705-025-06237-1
PMID:39934572
Abstract

A novel Vibrio parahaemolyticus phage, designated vB_VpaM_PVP-XSN (hereafter referred to as PVP-XSN), was isolated from costal soil. PVP-XSN has a polyhedral head (54.1 ± 11.1 nm in diameter) and a long contractile tail (110.5 ± 3.2 nm in length). The phage exhibits rapid adsorption and has a burst size of 167 plaque-forming units (PFU) per cell. Furthermore, PVP-XSN shows tolerance to temperatures ranging from 20°C to 50°C and pH levels between 5 and 11. Its linear double-stranded DNA genome is 45,651 base pairs in length and contains 66 putative open reading frames (ORFs). Comparative genomic analysis suggests that PVP-XSN represents a new genus of viruses.

摘要

从沿海土壤中分离出一种新型副溶血性弧菌噬菌体,命名为vB_VpaM_PVP-XSN(以下简称PVP-XSN)。PVP-XSN具有多面体头部(直径54.1±11.1纳米)和长收缩尾(长度110.5±3.2纳米)。该噬菌体表现出快速吸附,每个细胞的爆发量为167个噬菌斑形成单位(PFU)。此外,PVP-XSN对20°C至50°C的温度和5至11的pH水平具有耐受性。其线性双链DNA基因组长度为45,651个碱基对,包含66个推定的开放阅读框(ORF)。比较基因组分析表明,PVP-XSN代表一种新的病毒属。

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