Zhang Jiexiu, Wang Zijie, Xu Zhen, Han Zhijian, Tao Jun, Lu Pei, Huang Zhengkai, Zhou Wanli, Zhao Chunchun, Tan Ruoyun, Gu Min
Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China (mainland).
Ann Transplant. 2016 Oct 4;21:611-618. doi: 10.12659/aot.899263.
BACKGROUND Chronic allograft dysfunction (CAD) is the major factor endangering the long-term allograft survival in kidney transplantation. The mechanisms of CAD remain unclear. MATERIAL AND METHODS A total of 64 renal transplant recipients were enrolled in our study and divided into a stable group and CAD group according to their allograft function. A group of 32 normal controls (healthy volunteers) were also included. An ELISA was used to detect serum interleukin-33 (IL-33), IL-2, IL-4, IL-10, IL-17, and interferon-gamma (IFN-γ). Flow cytometry was performed to measure the percentage of CD3+CD4+ and CD3+CD8+ T cells in the peripheral blood from the three patient groups. The correlations among the study indexes were also analyzed using Pearson's method. RESULTS Levels of serum IL-33 was significantly higher in CAD patients than recipients with stable allograft function. Moreover, serum IL-2, IL-4, and IL-10 also increased statistically in patients with CAD. In addition, significant differences were observed in CD4+ T cells and the ratio of CD4+ and CD8+ T cells between CAD and stable patients. CONCLUSIONS Serum upregulated IL-33 could contribute to the pathogenesis of CAD in kidney transplant recipients.
背景 慢性移植肾失功（CAD）是危及肾移植长期存活的主要因素。CAD的发病机制尚不清楚。材料与方法 本研究共纳入64例肾移植受者，根据移植肾功能分为稳定组和CAD组。还纳入了一组32名正常对照者（健康志愿者）。采用酶联免疫吸附测定法（ELISA）检测血清白细胞介素-33（IL-33）、IL-2、IL-4、IL-10、IL-17和干扰素-γ（IFN-γ）。运用流式细胞术检测三组患者外周血中CD3+CD4+和CD3+CD8+ T细胞的百分比。采用Pearson法分析研究指标之间的相关性。结果 CAD患者血清IL-33水平显著高于移植肾功能稳定的受者。此外，CAD患者血清IL-2、IL-4和IL-10水平也有统计学意义的升高。另外，CAD患者与移植肾功能稳定患者在CD4+ T细胞以及CD4+与CD8+ T细胞比值方面存在显著差异。结论 血清中上调的IL-33可能参与肾移植受者CAD的发病机制。
