Held Manuel, Engelke Anne-Sophie, Tolzmann Dascha Sophie, Rahmanian-Schwarz Afshin, Schaller Hans-Eberhard, Rothenberger Jens
BG-Trauma Center, Eberhard Karls University, Department of Plastic and Hand Surgery, Reconstructive and Aesthetic Surgery, Tuebingen, Germany.
HELIOS Klinikum Wuppertal GmbH, University Hospital Witten/Herdecke, Germany, Department of Plastic and Hand Surgery, Reconstructive and Aesthetic Surgery, Wuppertal, Germany.
Wounds. 2016 Sep;28(9):334-340.
There is a high prevalence of superficial wounds such as partial-thickness burns. Treatment of these wounds frequently includes temporary application of wound dressings. The aim of this study was to compare a newly developed collagen matrix with commonly used temporary skin dressings for treatment of partial-thickness skin defects.
Through a skin dermatome, 42 standardized superficial skin defects were generated on the back of 28 adult male Lewis rats. The wounds were treated with a synthetic wound dressing (Suprathel, Polymedics Innovations Inc, Woodstock, GA) (n = 14), a biosynthetic skin dressing (Biobrane, Smith & Nephew, Hull, UK) (n = 14), or a newly developed bovine collagen matrix, Collagen Cell Carrier (Viscofan BioEngineering, Weinheim, Germany) (n = 14). Biomechanical properties of the skin were determined and compared every 10 days over a 3-month period of using the Cutometer MPA 580 (Courage + Khazaka Electronic GmbH, Cologne, Germany).
As opposed to healthy skin, statistically significant differences were detected between days 10 and 30, and between days 60 and 80, for calculated elasticity (Ue), firmness of skin (R0), and overall elasticity (R8). After 3 months, no statistically significant differences in skin elasticity were detected between the different wound dressings.
The presented results give an opportunity to compare the wound dressings used for treatment with respect to skin elasticity and reveal the potential of the bovine collagen matrix in the treatment of superficial skin defects; therefore the results facilitate further evaluation of collagen matrix in surgical applications and regenerative medicine.
诸如浅度烧伤之类的浅表伤口非常普遍。这些伤口的治疗通常包括临时使用伤口敷料。本研究的目的是比较一种新开发的胶原蛋白基质与常用的临时皮肤敷料在治疗浅度皮肤缺损方面的效果。
通过皮肤切片机，在28只成年雄性Lewis大鼠的背部制造了42个标准化的浅表皮肤缺损。伤口分别用合成伤口敷料（Suprathel，Polymedics Innovations Inc，伍德斯托克，佐治亚州）（n = 14）、生物合成皮肤敷料（Biobrane，施乐辉公司，赫尔，英国）（n = 14）或新开发的牛胶原蛋白基质胶原细胞载体（Viscofan BioEngineering，魏因海姆，德国）（n = 14）进行治疗。在使用Cutometer MPA 580（Courage + Khazaka Electronic GmbH，科隆，德国）的3个月期间，每10天测定并比较皮肤的生物力学特性。
与健康皮肤相比，在第10天和第30天之间以及第60天和第80天之间，计算得出的弹性（Ue）、皮肤硬度（R0）和整体弹性（R8）存在统计学上的显著差异。3个月后，不同伤口敷料之间在皮肤弹性方面未检测到统计学上的显著差异。
所呈现的结果为比较用于治疗的伤口敷料在皮肤弹性方面提供了机会，并揭示了牛胶原蛋白基质在治疗浅表皮肤缺损方面的潜力；因此这些结果有助于进一步评估胶原蛋白基质在外科手术应用和再生医学中的作用。
