Goihman-Yahr M, Rothenberg A, Bretaña A, Istúriz G, Rosquete R, Avila-Millán E, Viloria N, Saavedra de Borges N, Carrasquero M, Pérez de Fernández B
Section of Immunology I, Instituto de Biomedicina, Central University of Venezuela, Caracas.
Mycopathologia. 1989 Apr;106(1):53-8. doi: 10.1007/BF00436927.
We previously described an in vitro assay showing that neutrophils (PMNs) from patients with paracoccidioidomycosis (PARA) have a specific digestive deficiency against suspensions of live Paracoccidioides brasiliensis. We now report that this defect is equally detectable against autoclaved, but not Amphotericin B-killed P. brasiliensis. The use of autoclaved suspensions facilitates the use of our in vitro assay. It might allow the development of an in vitro intradermal test for digestion of fungi. Differential digestive ability of phagocytes against live (or autoclaved) and Amphotericin-B killed fungi is of conceptual interest. It may be relevant in understanding therapeutic effect of Amphotericin B.
我们之前描述了一种体外试验，该试验表明，副球孢子菌病（PARA）患者的中性粒细胞（PMNs）对巴西副球孢子菌的活悬液具有特定的消化缺陷。我们现在报告，这种缺陷在针对高压灭菌的巴西副球孢子菌时同样可检测到，但在针对两性霉素B灭活的巴西副球孢子菌时则检测不到。使用高压灭菌的悬液便于我们进行体外试验。这可能有助于开发一种用于真菌消化的体外皮内试验。吞噬细胞对活的（或高压灭菌的）真菌以及两性霉素B灭活的真菌的不同消化能力具有理论研究价值。这可能与理解两性霉素B的治疗效果相关。
