Goihman-Yahr M, Essenfeld-Yahr E, de Albornoz M C, Yarzábal L, de Gómez M H, San Martín B, Ocanto A, Gil F, Convit J
Infect Immun. 1980 May;28(2):557-66. doi: 10.1128/iai.28.2.557-566.1980.
Selected functions of polymorphonuclear leukocytes were studied in patients with paracoccidioidomycosis (South American blastomycosis), in healthy control individuals, and in patients with diseases unrelated to paracoccidioidomycosis. Patients with paracoccidioidomycosis were also evaluated by standard immunological techniques. Phagocytosis and digestion of Paracoccidioides brasiliensis yeastlike cells in vitro was estimated by an original method. It was based on the appearance of phagocytosed P. brasiliensis in preparations stained by a modification of the Papanicolaou method and examined with phase-contrast optics. Interpretation of such findings was confirmed by electron microscopy. Two strains of P. brasiliensis were used. Strain 8506 was freshly isolated from a patient. Strain Pb9 was known to be nonpathogenic and to have a peculiar cell wall composition. Yeastlike cells of the Pb9 strain were digested significantly better than those of strain 8506. A higher number of leukocytes per fungus cells led to a higher proportion of digested P. brasiliensis. Leukocytes from patients with paracoccidioidomycosis phagocytosed the fungus in a normal way, but had a significant lower ability to digest it in vitro. When individual cases were analyzed, there was an excellent correlation between clinical evolution and digestive ability of polymorphonuclear leukocytes. There was good correlation between both of these and immunological parameters. Leukocytes from all groups behaved comparably in tests of general leukocyte function and in their abilities to kill and digest Candida albicans. Our results indicate that, as a group, polymorphonuclear leukocytes from patients with paracoccidioidomycosis had a significant, rather specific, defect in their in vitro digestive capacity against phagocytosed P. brasiliensis. There was also an inverse correlation between strain pathogenicity and its susceptibility to in vitro digestion by polymorphonuclear leukocytes. Our findings are concordant with and relevant to clinical reality.
对类球孢子菌病（南美芽生菌病）患者、健康对照个体以及与类球孢子菌病无关疾病的患者，研究了多形核白细胞的特定功能。还采用标准免疫学技术对类球孢子菌病患者进行了评估。通过一种原创方法估计体外巴西副球孢子菌酵母样细胞的吞噬作用和消化情况。该方法基于经改良巴氏染色法染色并采用相差显微镜检查的制剂中被吞噬的巴西副球孢子菌的外观。通过电子显微镜证实了此类发现的解释。使用了两株巴西副球孢子菌。8506菌株是从一名患者身上新鲜分离得到的。已知Pb9菌株无致病性且具有独特的细胞壁组成。Pb9菌株的酵母样细胞比8506菌株的酵母样细胞消化得明显更好。每个真菌细胞的白细胞数量越多，巴西副球孢子菌的消化比例越高。类球孢子菌病患者的白细胞以正常方式吞噬真菌，但体外消化能力明显较低。对个别病例进行分析时，多形核白细胞的临床进展与消化能力之间存在极佳的相关性。这两者与免疫参数之间均存在良好的相关性。所有组的白细胞在一般白细胞功能测试以及杀死和消化白色念珠菌的能力方面表现相当。我们的结果表明，作为一个群体，类球孢子菌病患者的多形核白细胞在体外对吞噬的巴西副球孢子菌的消化能力方面存在显著的、相当特异的缺陷。菌株致病性与其对多形核白细胞体外消化的敏感性之间也存在负相关。我们的发现与临床实际相符且相关。
