Goihman-Yahr M, Essenfeld-Yahr E, Albornoz M C, Yarzábal L, de Gómez M H, San Martín B, Ocanto A, Convit J
J Clin Microbiol. 1979 Sep;10(3):365-70. doi: 10.1128/jcm.10.3.365-370.1979.
We describe a method by which phagocytosis and digestion of Paracoccidioides brasiliensis yeast cells by polymorphonuclear leukocytes or other phagocytic cells may be estimated. Suspensions of P. brasiliensis in its yeastlike phase were sonicated, counted, and incubated with known numbers of peripheral blood polymorphonuclear leukocytes. At given intervals, cytocentrifuge droplets were stained by a variation of Papanicolaou's method. Stained preparations were examined with phase-contrast optics. Digested organisms showed total or partial disappearance of protoplasm. Green-stained cell walls resisted digestion. The proportion of digested cells as a function of time was estimated.
我们描述了一种方法，通过该方法可以估计多形核白细胞或其他吞噬细胞对巴西副球孢子菌酵母细胞的吞噬作用和消化情况。将处于酵母样期的巴西副球孢子菌悬浮液进行超声处理、计数，并与已知数量的外周血多形核白细胞一起孵育。在给定的时间间隔，通过改良的巴氏染色法对细胞离心涂片进行染色。用相差显微镜检查染色后的标本。被消化的生物体显示原生质全部或部分消失。绿色染色的细胞壁抵抗消化。估计消化细胞的比例随时间的变化情况。
