Key Laboratory of Radiopharmaceuticals, Ministry of Education, College of Chemistry, Beijing Normal University, Beijing 100875, P. R. China.
Department of Applied Chemistry, School of Engineering, The University of Tokyo, Tokyo 113-8654, Japan.
Nanoscale. 2016 Sep 15;8(36):16172-16176. doi: 10.1039/c6nr04103e.
We present an efficient strategy through surface functionalization to build a single silicon nanowire field-effect transistor-based biosensor that is capable of directly detecting protein adsorption/desorption at the single-event level. The step-wise signals in real-time detection of His-tag F-ATPases demonstrate a promising electrical biosensing approach with single-molecule sensitivity, thus opening up new opportunities for studying single-molecule biophysics in broad biological systems.
我们提出了一种通过表面功能化构建单根硅纳米线场效应晶体管生物传感器的有效策略，该传感器能够直接在单事件水平上检测蛋白质的吸附/解吸。His-tag F-ATPases 的实时检测中的逐步信号表明，该电生物传感方法具有单分子灵敏度，为在广泛的生物系统中研究单分子生物物理学提供了新的机会。
