Molecular characterization of and phylogenetic analysis based on mitochondrial (nicotiamide adenine dinucleotide dehydrogenase subunit I and cytochrome oxidase subunit I) genes from the North-East of Iran.

AIM

Fascioliasis is one of the most zoonotic diseases with global extension. As the epidemiological distribution of may lead to various genetic patterns of the parasite, the aim of this study is to identify based on spermatogenesis, and phylogenetic analysis using mitochondrial (nicotiamide adenine dinucleotide dehydrogenase subunit I [ND1] and cytochrome oxidase subunit I) gene marker.

MATERIALS AND METHODS

In this study, 90 collected from 30 cattle at slaughterhouse located in three different geographical locations in the North-East of Iran were evaluated based on spermatogenetic ability and internal transcribed spacer 1 gene restriction fragment length polymorphism pattern. Genetic diversity and phylogenetic relationship using mtDNA gene marker for the isolates from the North-East of Iran, and other countries were then analyzed.

RESULTS

Partial sequences of mtDNA showed eight haplotypes in both genes. The phylogenic analysis using neighbor joining as well as maximum likelihood methods showed similar topologies of trees. Pairwise fixation index between different populations calculated from the nucleotide data set of ND1 gene are statistically significant and show the genetic difference.

CONCLUSION

found in this region of Iran has different genetic structures through the other populations in the world.