Reaghi Saber, Haghighi Ali, Harandi Majid Fasihi, Spotin Adel, Arzamani Kourosh, Rouhani Soheila
Department of Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Research Center for Hydatid disease in Iran, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
Vet World. 2016 Sep;9(9):1034-1038. doi: 10.14202/vetworld.2016.1034-1038. Epub 2016 Sep 29.
Fascioliasis is one of the most zoonotic diseases with global extension. As the epidemiological distribution of may lead to various genetic patterns of the parasite, the aim of this study is to identify based on spermatogenesis, and phylogenetic analysis using mitochondrial (nicotiamide adenine dinucleotide dehydrogenase subunit I [ND1] and cytochrome oxidase subunit I) gene marker.
In this study, 90 collected from 30 cattle at slaughterhouse located in three different geographical locations in the North-East of Iran were evaluated based on spermatogenetic ability and internal transcribed spacer 1 gene restriction fragment length polymorphism pattern. Genetic diversity and phylogenetic relationship using mtDNA gene marker for the isolates from the North-East of Iran, and other countries were then analyzed.
Partial sequences of mtDNA showed eight haplotypes in both genes. The phylogenic analysis using neighbor joining as well as maximum likelihood methods showed similar topologies of trees. Pairwise fixation index between different populations calculated from the nucleotide data set of ND1 gene are statistically significant and show the genetic difference.
found in this region of Iran has different genetic structures through the other populations in the world.
肝片吸虫病是一种在全球广泛传播的人畜共患病。由于其流行病学分布可能导致寄生虫的各种遗传模式，本研究旨在基于精子发生以及使用线粒体（烟酰胺腺嘌呤二核苷酸脱氢酶亚基I [ND1]和细胞色素氧化酶亚基I）基因标记进行系统发育分析来鉴定[具体内容缺失]。
在本研究中，从位于伊朗东北部三个不同地理位置的屠宰场的30头牛身上采集了90份[具体内容缺失]，根据精子发生能力和内部转录间隔区1基因限制性片段长度多态性模式进行评估。然后分析了来自伊朗东北部以及其他国家的分离株使用线粒体DNA基因标记的遗传多样性和系统发育关系。
线粒体DNA的部分序列在两个基因中均显示出8种单倍型。使用邻接法以及最大似然法进行的系统发育分析显示出相似的树形拓扑结构。根据ND1基因的核苷酸数据集计算出的不同[具体内容缺失]群体之间的成对固定指数具有统计学意义，并显示出遗传差异。
在伊朗该地区发现的[具体内容缺失]与世界上其他[具体内容缺失]群体具有不同的遗传结构。
