Antonelli Antonella, Sfara Carla, Weber Oliver, Pison Ulrich, Manuali Elisabetta, Salamida Sonia, Magnani Mauro
Department of Biomolecular Sciences, University of Urbino Carlo Bo, Via Saffi 2, 61029 Urbino (PU), Italy.
Philips Medical Systems DMC GmbH, Röntgenstraβe 24-26, D-22335 Hamburg, Germany.
Nanomedicine (Lond). 2016 Nov;11(21):2781-2795. doi: 10.2217/nnm-2016-0216. Epub 2016 Oct 14.
The biomedical application of contrast agents based on superparamagnetic iron oxide nanoparticles is still limited because of their short intravascular half-life. The potential of red blood cells (RBCs) loaded with new ferucarbotran nanoparticles as magnetic contrast agents with longer blood retention time has been investigated.
MATERIALS & METHODS: Ferucarbotran was loaded into RBCs by a procedure of hypotonic dialysis and isotonic resealing. Ferucarbotran amounts encapsulated in RBCs were determined by NMR. The survival of ferucarbotran-loaded RBCs and bulk ferucarbotran was evaluated in the mouse bloodstream.
Blood retention time of these RBC constructs is longer (∼14 days) than the bulk ferucarbotran (∼1 h) with a slower Fe clearance from liver and spleen.
Ferucarbotran-loaded RBCs could be used as potential contrasting agents for diagnostic applications in MRI/magnetic particle imaging.
基于超顺磁性氧化铁纳米颗粒的造影剂在生物医学中的应用仍然受到限制，因为它们在血管内的半衰期较短。研究了负载新型 ferucarbotran 纳米颗粒的红细胞（RBC）作为具有更长血液保留时间的磁性造影剂的潜力。
通过低渗透析和等渗重封的方法将 ferucarbotran 负载到红细胞中。通过核磁共振（NMR）测定红细胞中包裹的 ferucarbotran 含量。在小鼠血液中评估负载 ferucarbotran 的红细胞和大量 ferucarbotran 的存活情况。
这些红细胞构建体的血液保留时间更长（约 14 天），比大量 ferucarbotran（约 1 小时）长，并且从肝脏和脾脏清除铁的速度较慢。
负载 ferucarbotran 的红细胞可作为潜在的造影剂用于 MRI/磁性粒子成像的诊断应用。
