Lin Jin, Wang Yan, Zou Ye-Qing, Chen Xin, Huang Bo, Liu Jing, Xu Yan-Mei, Li Jing, Zhang Jing, Yang Wei-Ming, Min Qing-Hua, Sun Fan, Li Shu-Qi, Gao Qiu-Fang, Wang Xiao-Zhong
Department of Clinical Laboratory, The Second Affiliated Hospital of Nanchang University, No. 1 Min De Road, Nanchang, 330006, China.
Department of Clinical Laboratory, The First Affiliated Hospital of Nanchang University, Nanchang, 330006, China.
Tumour Biol. 2016 Dec;37:15835–15845. doi: 10.1007/s13277-016-5410-6. Epub 2016 Oct 14.
MicroRNAs (miRNAs) have been found to play important regulatory roles in various physiological and pathological processes. MiRNAs also exhibit high stability and are present at high concentrations in human bodily fluids. Consequently, miRNAs may represent attractive and novel diagnostic biomarkers for certain clinical conditions. Recently, the capacity for extracellular vesicles, including microvesicles and exosomes, to carry miRNAs that participate in cell-to-cell communication has been described. In the present study, the miRNA expression patterns for three kinds of pleural effusions that were obtained from patients with pneumonia (group A), pulmonary tuberculosis (group B), and lung cancer (group C) were detected with high-throughput sequencing. When the expression levels of these miRNAs were compared among the three groups, three differentially expressed miRNAs were detected between groups A and B, while 27 differentially expressed miRNAs were detected between groups A and C. Notably, miR-378i was significantly elevated only in group B, while miR-205-5p and miR-200b were markedly increased only in group C (p < 0.01). Further studies are needed to confirm whether these differentially expressed miRNAs may serve as prospective diagnostic markers for pulmonary diseases.
微小RNA（miRNA）已被发现可在各种生理和病理过程中发挥重要的调节作用。miRNA还具有高稳定性，并且在人体体液中以高浓度存在。因此，miRNA可能是某些临床病症有吸引力的新型诊断生物标志物。最近，已经描述了包括微泡和外泌体在内的细胞外囊泡携带参与细胞间通讯的miRNA的能力。在本研究中，采用高通量测序检测了从肺炎患者（A组）、肺结核患者（B组）和肺癌患者（C组）获得的三种胸腔积液的miRNA表达模式。当比较这三组中这些miRNA的表达水平时，在A组和B组之间检测到3种差异表达的miRNA，而在A组和C组之间检测到27种差异表达的miRNA。值得注意的是，miR-378i仅在B组中显著升高，而miR-205-5p和miR-200b仅在C组中显著增加（p<0.01）。需要进一步研究来证实这些差异表达的miRNA是否可作为肺部疾病的潜在诊断标志物。
