Wang Yan, Xu Yan-Mei, Zou Ye-Qing, Lin Jin, Huang Bo, Liu Jing, Li Jing, Zhang Jing, Yang Wei-Ming, Min Qing-Hua, Li Shu-Qi, Gao Qiu-Fang, Sun Fan, Chen Qing-Gen, Zhang Lei, Jiang Yu-Huan, Deng Li-Bin, Wang Xiao-Zhong
Department of Clinical Laboratory, The Second Affiliated Hospital of Nanchang University, Jiangxi Department of Hematology, The Affiliated Hospital of Guizhou Medical University, Guizhou The Key Laboratory of Molecular Medicine, The Second Affiliated Hospital of Nanchang University Department of Clinical Laboratory, The First Affiliated Hospital of Nanchang University Institute of Translational Medicine, Nanchang University, Jiangxi, China.
Medicine (Baltimore). 2017 Nov;96(44):e8361. doi: 10.1097/MD.0000000000008361.
Pleural effusion (PE) is a common clinical complication of many pulmonary and systemic diseases, including lung cancer and tuberculosis. Nevertheless, there is no clinical effective biomarker to identify the cause of PE. We attempted to investigate differential expressed exosomal miRNAs in PEs of lung adenocarcinoma (APE), tuberculous (TPE), and other benign lesions (NPE) by using deep sequencing and quantitative polymerase chain reaction (qRT-PCR). As a result, 171 differentiated miRNAs were observed in 3 groups of PEs, and 11 significantly differentiated exosomal miRNAs were validated by qRT-PCR. We identified 9 miRNAs, including miR-205-5p, miR-483-5p, miR-375, miR-200c-3p, miR-429, miR-200b-3p, miR-200a-3p, miR-203a-3p, and miR-141-3p which were preferentially represented in exosomes derived from APE when compared with TPE or NPE, while 3 miRNAs, including miR-148a-3p, miR-451a, and miR-150-5p, were differentially expressed between TPE and NPE. These different miRNAs profiles may hold promise as biomarkers for differential diagnosis of PEs with more validation based on larger cohorts.
胸腔积液（PE）是许多肺部和全身性疾病（包括肺癌和肺结核）常见的临床并发症。然而，目前尚无临床有效的生物标志物来确定PE的病因。我们试图通过深度测序和定量聚合酶链反应（qRT-PCR）研究肺腺癌（APE）、结核性（TPE）和其他良性病变（NPE）所致胸腔积液中差异表达的外泌体微小RNA（miRNA）。结果，在3组胸腔积液中观察到171种差异miRNA，通过qRT-PCR验证了11种显著差异的外泌体miRNA。我们鉴定出9种miRNA，包括miR-205-5p、miR-483-5p、miR-375、miR-200c-3p、miR-429、miR-200b-3p、miR-200a-3p、miR-203a-3p和miR-141-3p，与TPE或NPE相比，这些miRNA在APE来源的外泌体中优先表达，而3种miRNA，包括miR-148a-3p、miR-451a和miR-150-5p，在TPE和NPE之间差异表达。基于更大队列的更多验证，这些不同的miRNA谱可能有望成为胸腔积液鉴别诊断的生物标志物。
