Hagel Christian, Buslei Rolf, Buchfelder Michael, Fahlbusch Rudolf, Bergmann Markus, Giese Armin, Flitsch Jörg, Lüdecke Dieter K, Glatzel Markus, Saeger Wolfgang
Institute of Neuropathology, University Medical Center Hamburg-Eppendorf, Martinistr. 52, 20246, Hamburg, Germany.
Department of Neuropathology, Friedrich-Alexander University Erlangen-Nürnberg, Schwabachanlage 6, 91054, Erlangen, Germany.
Pituitary. 2017 Apr;20(2):211-217. doi: 10.1007/s11102-016-0762-x.
To analyse the antigen expression profiles of 27 cases of pituicytoma, spindle cell oncocytoma, and granular cell tumour of the sellar region concerning a common pituicytic origin of neoplastic cells.
Material from 12 female and 15 male patients (13 granular cell tumours of the sellar region, 10 pituicytomas, four spindle cell oncocytomas) collected in the German Registry of Pituitary Tumours between 1993 and 2015 was re-evaluated according to the current WHO classification of tumours of the central nervous system and supplementary immunohistochemistry including S100-protein, CD56, CD68, thyroid transcription factor-1 (TTF-1), and Ki-67 was performed.
S100-protein was detected in all 27 tumours and TTF-1 in all 16 tumours that were assessed. Vimentin was expressed in all 13 cases investigated whereas broad spectrum cytokeratin was not detected in any of 14 evaluated cases. GFAP was observed in nine out of 21 cases. 15 out of 17 investigated lesions showed some CD68 expression and five out of 14 cases were labelled with CD56 antibodies. Proliferative activity did not differ significantly between the three tumour subgroups although one primary and one recurrent pituicytoma showed exceptionally high Ki-67-proliferation indices of 15.3 and 12.7 %, respectively (means: granular cell tumour of the sellar region 2.0 %, pituicytoma 2.8 %, spindle cell oncocytoma 2.7 %).
The study confirms and expands earlier data and is in line with the notion that the three tumour types are variants of pituicytoma.
分析27例鞍区垂体细胞瘤、梭形细胞嗜酸细胞瘤和颗粒细胞瘤的抗原表达谱，探讨肿瘤细胞共同的垂体细胞起源。
对1993年至2015年间德国垂体肿瘤登记处收集的12例女性和15例男性患者的材料（13例鞍区颗粒细胞瘤、10例垂体细胞瘤、4例梭形细胞嗜酸细胞瘤），根据世界卫生组织中枢神经系统肿瘤的现行分类进行重新评估，并进行包括S100蛋白、CD56、CD68、甲状腺转录因子-1（TTF-1）和Ki-67的补充免疫组化检测。
在所有27例肿瘤中均检测到S100蛋白，在所有16例评估的肿瘤中均检测到TTF-1。波形蛋白在所有13例研究病例中均有表达，而在14例评估病例中均未检测到广谱细胞角蛋白。在21例病例中有9例观察到胶质纤维酸性蛋白（GFAP）。在17例研究病变中有15例显示出一定程度的CD68表达，在14例病例中有5例被CD56抗体标记。尽管1例原发性和1例复发性垂体细胞瘤的Ki-67增殖指数分别异常高，为15.3%和12.7%（平均值：鞍区颗粒细胞瘤2.0%，垂体细胞瘤2.8%，梭形细胞嗜酸细胞瘤2.7%），但三个肿瘤亚组之间的增殖活性无显著差异。
本研究证实并扩展了早期数据，与这三种肿瘤类型是垂体细胞瘤变体的观点一致。
