Xia Sha, Chen Gan, Wang Bo, Yin Yujing, Sun Zhenwei, Zhao Jingxiang, Li Penglong, Zhao Lian, Zhou Hong
Beijing Institute of Transfusion Medicine, Beijing Key Laboratory of Blood Safety and Supply Technologies, No. 27 Taiping Road, Haidian, Beijing, China.
Mediators Inflamm. 2016;2016:3549207. doi: 10.1155/2016/3549207. Epub 2016 Sep 26.
RBCs undergo numerous changes during storage and stored RBCs may induce adverse effects, ultimately resulting in organ injury in transfusion recipients. We tested the hypothesis that the addition of SP to stored RBCs would improve the quality of the stored RBCs and mitigate liver injury after transfusion in a murine model. RBCs were harvested from C57BL/6J mice and stored for 14 days in CPDA-1 containing either a solution of SP in saline or saline alone. Haemolysis, the 24-hour posttransfusion recovery, the oxygen-carrying capacity, and the SOD activity of stored RBCs were evaluated. The plasma biochemistry, hepatic MDA level, MPO activity, IL-6, TNF- concentrations, and histopathology were measured two hours after the transfusion of stored RBCs. Compared with RBCs stored in CPDA-1 and saline, the addition of SP to stored RBCs restored their oxygen-carrying capacity and SOD activity, reduced the AST activity, BUN concentrations, and LDH activity in the plasma, and decreased the MDA level, MPO activity, and concentrations of IL-6 and TNF- in the liver. These data indicate that the addition of SP to RBCs during storage has a beneficial effect on storage lesions and subsequently alleviates liver injury after the transfusion of stored RBCs .
红细胞在储存过程中会发生许多变化，储存的红细胞可能会产生不良反应，最终导致输血受者的器官损伤。我们测试了一个假设，即在小鼠模型中，向储存的红细胞中添加SP可以提高储存红细胞的质量，并减轻输血后的肝损伤。从C57BL/6J小鼠中采集红细胞，并在含有SP生理盐水溶液或仅含生理盐水的CPDA-1中储存14天。评估储存红细胞的溶血、输血后24小时恢复、携氧能力和超氧化物歧化酶（SOD）活性。在输注储存红细胞两小时后，测量血浆生化指标、肝脏丙二醛（MDA）水平、髓过氧化物酶（MPO）活性、白细胞介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）浓度以及组织病理学。与储存在CPDA-1和生理盐水中的红细胞相比，向储存的红细胞中添加SP可恢复其携氧能力和SOD活性，降低血浆中的天冬氨酸转氨酶（AST）活性、尿素氮（BUN）浓度和乳酸脱氢酶（LDH）活性，并降低肝脏中的MDA水平、MPO活性以及IL-6和TNF-α的浓度。这些数据表明，在储存期间向红细胞中添加SP对储存损伤有有益影响，并随后减轻输注储存红细胞后的肝损伤。
