Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China.
Hubei Engineering Technology Research Center for Aquatic Animal Diseases Control and Prevention, Wuhan 430070, China.
Int J Mol Sci. 2019 Jan 17;20(2):389. doi: 10.3390/ijms20020389.
is a common group of probiotics that have been widely used in the feed industry as they can increase host resistance to pathogens and balance the immune response. However, the regulatory mechanism of on the host immune system remains unclear in teleosts. In this study, we isolated and enriched dendritic cells from white blood cells (WBCs), and then stimulated them with . Morphological features, specific biological functions, and authorized functional molecular markers were used in the identification of dendritic cells. Subsequently, we collected stimulated cells at 0, 4, and 18 h, and then constructed and sequenced the transcriptomic libraries. A transcriptome analysis showed that 2557 genes were up-regulated and 1708 were down-regulated at 4 h compared with the control group (|Fold Change| ≥ 4), and 1131 genes were up-regulated and 1769 were down-regulated between the cells collected at 18 h and 4 h (|Fold Change| ≥ 4). Gene Ontology (GO) annotations suggested many differentially expressed genes (DEGs) ( < 0.05 and |Fold Change| ≥ 4) were involved in immune-related biological functions including immune system progress, cytokine receptor binding, and cytokine binding. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that the cytokine⁻cytokine receptor interaction pathways were significantly enriched at both time points ( < 0.05), which may play a key role in the response to stimulation. Furthermore, mRNA expression level examination of several pro-inflammatory cytokines and anti-inflammatory cytokines genes by quantitative real-time polymerase chain reaction (qRT-PCR) indicated that their expressions can be significantly increased in , which suggest that can balance immune response and tolerance. This study provides dendritic cell (DC)-specific transcriptome data in grass carp by stimulation, allowing us to illustrate the molecular mechanism of the DC-mediated immune response triggered by probiotics in grass carp.
是一类常见的益生菌，已广泛应用于饲料工业，因为它们可以提高宿主对病原体的抵抗力并平衡免疫反应。然而，在硬骨鱼类中，关于益生菌对宿主免疫系统的调节机制尚不清楚。在本研究中，我们从白细胞（WBC）中分离和富集了树突状细胞，然后用刺激它们。使用形态特征、特定的生物学功能和授权的功能分子标记来鉴定树突状细胞。随后，我们在 0、4 和 18 h 收集刺激细胞，并构建和测序转录组文库。转录组分析表明，与对照组相比，4 h 时有 2557 个基因上调，1708 个基因下调（|Fold Change|≥4），而在 18 h 和 4 h 收集的细胞之间有 1131 个基因上调，1769 个基因下调（|Fold Change|≥4）。基因本体论（GO）注释表明，许多差异表达基因（DEG）（ < 0.05 和 |Fold Change|≥4）参与了与免疫相关的生物学功能，包括免疫系统进程、细胞因子受体结合和细胞因子结合。京都基因与基因组百科全书（KEGG）通路分析表明，两个时间点细胞因子⁻细胞因子受体相互作用通路均显著富集（ < 0.05），这可能在刺激反应中发挥关键作用。此外，通过定量实时聚合酶链反应（qRT-PCR）对几种促炎细胞因子和抗炎细胞因子基因的 mRNA 表达水平进行检测，结果表明在 刺激下其表达水平可显著升高，提示 可平衡免疫反应和耐受。本研究通过 刺激提供了草鱼树突状细胞的特异性转录组数据，使我们能够阐明益生菌在草鱼中触发的树突状细胞介导的免疫反应的分子机制。
