James S W, Lefebvre P A
Department of Genetics and Cell Biology, University of Minnesota, St. Paul 55108-1095.
Curr Genet. 1989 Jun;15(6):443-52. doi: 10.1007/BF00376802.
Three independent pleiotropic drug-resistance (pdr) mutants were isolated by selecting for resistance to the anti-microtubule herbicides amiprophos-methyl (APM) and oryzalin (ORY). These three mutants and a previously isolated mutant, ani1 (anisomycin resistance), were semi-dominant in heterozygous diploids, and they displayed varying degrees of resistance to structurally and functionally unrelated inhibitors such as cycloheximide, cryptopleurine, emetine, atrazine, and nonidet P-40. Linkage analysis and genetic mapping suggested that three of the four mutants, including ani1, define a single locus, here named pdr1. The fourth mutant defined a new locus, pdr2, which is located on the left arm of linkage group VI. One pdr1 mutant exhibited unusual genetic interactions, including enhanced ts-lethality and synergistic increases in drug resistance, when combined with pdr2-1 and with herbicide-resistant alleles of three other genes.
通过筛选对抗微管除草剂甲基胺草磷（APM）和安磺灵（ORY）的抗性，分离出了三个独立的多效性药物抗性（pdr）突变体。这三个突变体和一个先前分离的突变体ani1（茴香霉素抗性）在杂合二倍体中呈半显性，并且它们对结构和功能不相关的抑制剂如环己酰亚胺、隐品碱、吐根碱、莠去津和诺乃洗涤剂P - 40表现出不同程度的抗性。连锁分析和基因定位表明，包括ani1在内的四个突变体中的三个定义了一个单一基因座，这里命名为pdr1。第四个突变体定义了一个新的基因座pdr2，它位于连锁群VI的左臂上。当与pdr2 - 1以及其他三个基因的除草剂抗性等位基因结合时，一个pdr1突变体表现出异常的遗传相互作用，包括增强的温度敏感性致死性和耐药性的协同增加。
