Ara Katayoun Mahdavi, Raofie Farhad
Department of Applied Chemistry, Faculty of Chemistry, Shahid Beheshti University, G. C., Tehran, Iran.
J Food Sci Technol. 2016 Jul;53(7):3113-3121. doi: 10.1007/s13197-016-2284-y. Epub 2016 Jul 22.
Essential oils and volatile components of pomegranate ( L.) peel of the Malas variety from Meybod, Iran, were extracted using supercritical fluid extraction (SFE) and hydro-distillation methods. The experimental parameters of SFE that is pressure, temperature, extraction time, and modifier (methanol) volume were optimized using a central composite design after a (2) fractional factorial design. Detailed chemical composition of the essential oils and volatile components obtained by hydro-distillation and optimum condition of the supercritical CO extraction were analyzed by GC-MS, and seventy-three and forty-six compounds were identified according to their retention indices and mass spectra, respectively. The optimum SFE conditions were 350 atm pressure, 55 °C temperature, 30 min extraction time, and 150 µL methanol. Results showed that oleic acid, palmitic acid and (-)-Borneol were major compounds in both extracts. The optimum extraction yield was 1.18 % (w/w) for SFE and 0.21 % (v/w) for hydro-distillation.
采用超临界流体萃取（SFE）和水蒸馏法，对来自伊朗迈博德的马拉斯品种石榴（L.）果皮中的精油和挥发性成分进行了提取。在二水平部分因子设计之后，采用中心复合设计对SFE的实验参数（即压力、温度、萃取时间和改性剂（甲醇）体积）进行了优化。通过气相色谱 - 质谱联用（GC-MS）分析了水蒸馏法获得的精油和挥发性成分的详细化学组成以及超临界CO₂萃取的最佳条件，分别根据保留指数和质谱鉴定出了73种和46种化合物。最佳SFE条件为350个大气压、55℃温度、30分钟萃取时间和150微升甲醇。结果表明，油酸、棕榈酸和（-）-冰片是两种提取物中的主要化合物。SFE的最佳萃取率为1.18%（w/w），水蒸馏法的最佳萃取率为0.21%（v/w）。
