Haggarty Jennifer, Burgess Karl Ev
Polyomics, University of Glasgow, 211 Wolfson Wohl Translational Cancer Research Centre, Garscube Campus, Glasgow G61 1QH, UK.
Polyomics, University of Glasgow, 211 Wolfson Wohl Translational Cancer Research Centre, Garscube Campus, Glasgow G61 1QH, UK.
Curr Opin Biotechnol. 2017 Feb;43:77-85. doi: 10.1016/j.copbio.2016.09.006. Epub 2016 Oct 20.
The metabolome is the complete complement of metabolites (small organic biomolecules). In order to comprehensively understand the effect of stimuli on a biological system, it is important to detect as many of the metabolites within that system as possible. This review briefly describes some new advances in liquid and gas chromatography to improve coverage of the metabolome, including the serial combination of two columns in tandem, column switching and different variations of two-dimensional chromatography. Supercritical fluid chromatography could provide complimentary data to liquid and gas chromatography. Although there have been many recent advancements in the field of metabolomics, it is evident that a combination, rather than a single method, is required to approach full coverage of the metabolome.
代谢组是代谢物（有机小分子生物分子）的完整集合。为了全面了解刺激对生物系统的影响，尽可能多地检测该系统内的代谢物非常重要。本综述简要介绍了液相色谱和气相色谱的一些新进展，以提高代谢组的覆盖范围，包括两根色谱柱串联、柱切换以及二维色谱的不同变体。超临界流体色谱可为液相色谱和气相色谱提供补充数据。尽管代谢组学领域最近有许多进展，但显然需要多种方法结合，而非单一方法，才能实现对代谢组的全面覆盖。
