Ministry of Education Key Laboratory of Synthetic and Natural Functional Molecule Chemistry, College of Chemistry & Materials Science, Northwest University , Xi'an, Shaanxi 710127, P. R. China.
School of Materials and Chemical Engineering, Xi'an Technological University , Xi'an 710032, Shaanxi P. R. China.
Anal Chem. 2016 Nov 15;88(22):11253-11260. doi: 10.1021/acs.analchem.6b04087. Epub 2016 Nov 4.
A broad-spectrum fluorescent probe, which can be applied to monitoring HS in various biological systems, has been rationally designed and synthesized. This specific probe was applied to localize the endogenous HS in living Raw264.7 macrophage cells, HepG2 cells, and H9C2 cells. At the same time, the probe has successfully visualized CBS- and CSE-induced endogenous HS production and monitored CBS and CSE activity in H9C2 cells. This probe could serve as a powerful molecular imaging tool to further explore the physiological function and the molecular mechanisms of endogenous HS in living animal systems.
一种广谱荧光探针，可应用于各种生物体系中 HS 的监测，已被合理设计和合成。该探针被用于对活 Raw264.7 巨噬细胞、HepG2 细胞和 H9C2 细胞中的内源性 HS 进行定位。同时，该探针成功地可视化了 CBS 和 CSE 诱导的内源性 HS 的产生，并监测了 H9C2 细胞中 CBS 和 CSE 的活性。该探针可以作为一种强大的分子成像工具，用于进一步探索内源性 HS 在活体动物系统中的生理功能和分子机制。
