Yadav Pankaj, Chaudhary Sarika, Saxena Rajendra K, Talwar Sangeeta, Yadav Sudha
Senior Resident Associate Professor, Department of Conservative Dentistry and Endodontics, Maulana Azad Institute of Dental Sciences, New Delhi, India.
Associate Professor, Department of Conservative Dentistry and Endodontics, Maulana Azad Institute of Dental Sciences, New Delhi, India.
J Clin Exp Dent. 2017 Mar 1;9(3):e361-e367. doi: 10.4317/jced.53210. eCollection 2017 Mar.
Bacterial biofilms formed on the root canal wall are often difficult to remove. This study aimed to evaluate the cytotoxic effect and antibacterial efficacy of chitosan when used as root canal irrigant against and biofilm formed on tooth substrate.
The present study evaluated antibacterial effect of 0.25% Chitosan, 0.5% Chitosan, 2% chlorhexidine and 3% sodium hypochlorite against and . Agar-well diffusion methods, minimal inhibitory concentration tests and biofilm susceptibility assays were used to determine antibacterial activity. Teeth specimens were sectioned to obtain a standardized tooth length of 12mm. Specimens were inoculated with 10 mL of the freshly prepared suspension and for 4 weeks. The specimens were then instrumented with ProTaper rotary files F3 size. After irrigation with test solution, three sterile paper points were placed into one canal, left for 60 s and transferred to a test tube containing 1 mL of reduced transport fluid. The number of CFU in 1 mL was determined.
3-week biofilm qualitative assay showed complete inhibition of bacterial growth with 3% Sodium hypochlorite, 2% Chlorhexidine and Chitosan except saline, which showed presence of bacterial growth. Significant reduction of colony forming units (CFU)/mL was observed for the chitosan groups and the antibacterial activity of the chitosan groups was at par with 3% NaOCl and 2% Chlorhexidine. It was observed that the chitosan showed no cytotoxicity at 3mg/ml and 10% cytotoxicity at 6mg/ml.
The use of chitosan as a root canal irrigant might be an alternative considering the various undesirable properties of NaOCl and chlorhexidine. Biofilm, Candida albicans, Chitosan, Cytotoxicity, Enterococcus faecalis.
根管壁上形成的细菌生物膜通常难以清除。本研究旨在评估壳聚糖作为根管冲洗剂对在牙齿基质上形成的粪肠球菌和白色念珠菌生物膜的细胞毒性作用及抗菌效果。
本研究评估了0.25%壳聚糖、0.5%壳聚糖、2%氯己定和3%次氯酸钠对粪肠球菌和白色念珠菌的抗菌效果。采用琼脂孔扩散法、最低抑菌浓度试验和生物膜敏感性测定来确定抗菌活性。将牙齿标本切片以获得12毫米的标准化牙齿长度。标本接种10毫升新鲜制备的粪肠球菌悬液和白色念珠菌悬液4周。然后用ProTaper F3号旋转锉对标本进行预备。用测试溶液冲洗后，将三个无菌纸尖放入一个根管中，放置60秒，然后转移到含有1毫升稀释运输液的试管中。测定1毫升中的菌落形成单位（CFU）数量。
3周生物膜定性试验显示，除生理盐水显示有细菌生长外，3%次氯酸钠、2%氯己定和壳聚糖可完全抑制细菌生长。壳聚糖组的菌落形成单位（CFU）/毫升显著减少，壳聚糖组的抗菌活性与3%次氯酸钠和2%氯己定相当。观察到壳聚糖在3毫克/毫升时无细胞毒性，在6毫克/毫升时有10%的细胞毒性。
考虑到次氯酸钠和氯己定的各种不良特性，使用壳聚糖作为根管冲洗剂可能是一种替代方法。生物膜、白色念珠菌、壳聚糖、细胞毒性、粪肠球菌。
