Vinothkumar Thilla S, Rubin Mohamed I, Balaji Lakshmi, Kandaswamy Deivanayagam
Department of Conservative Dentistry and Endodontics, Faculty of Dental Sciences, Sri Ramachandra University, Chennai, Tamil Nadu, India.
J Conserv Dent. 2013 Mar;16(2):167-70. doi: 10.4103/0972-0707.108208.
Sodium hypochlorite is the most commonly used irrigant but it has disadvantage like high cytotoxicity. So there is a need to find an alternative to 5.25% Sodium hypochlorite against microorganism Enterococcus faecalis and Candida albicans. Literature has shown that these 5 extracts namely Terminalia chebula, Myristica frangrans, Aloe barbadensis, Curcuma longa and Azadaricta indica has good properties which can be used as a potential endodontic irrigant.
To evaluate the antimicrobial efficacy of various herbal extracts namely Curcuma longa (CL), Azadiracta indica (AI), Aloe barbadensis (AV), Myristica fragrans (MF) and Terminalia chebula (TC) as endodontic irrigant against Enterococcus faecalis and Candida albicans using real-time quantitative polymerase chain reaction (qPCR).
Eighty-four teeth were extracted and suspended with Enterococcus faecalis and Candida albicans. A preliminary study was first performed to determine the minimum inhibitory concentration of extracts. The irrigating groups were divided into five herbal groups and 2 control groups. After irrigating the teeth the remaining microbial load was determined using qPCR.
Statistical analysis was performed using Oneway Anova/Kruskal-Wallis test with post-hoc Tukey's HSD and was statistically significant (P < 0.05).
It was shown that Neem was highly efficient to 5.25% NaOCl in reducing Enterococcus faecalis and Candida albicans within the root canals when compared with other extracts.
Neem leaf extract has a significant antimicrobial efficacy against Enterococcus faecalis and Candida albicans compared to 5.25% sodium hypochlorite.
次氯酸钠是最常用的冲洗剂，但它有诸如高细胞毒性等缺点。因此，需要找到一种替代5.25%次氯酸钠的物质来对抗粪肠球菌和白色念珠菌。文献表明，诃子、肉豆蔻、库拉索芦荟、姜黄和印楝这5种提取物具有良好的特性，可作为潜在的牙髓冲洗剂。
使用实时定量聚合酶链反应（qPCR）评估姜黄（CL）、印楝（AI）、库拉索芦荟（AV）、肉豆蔻（MF）和诃子（TC）等各种草药提取物作为牙髓冲洗剂对粪肠球菌和白色念珠菌的抗菌效果。
拔除84颗牙齿，接种粪肠球菌和白色念珠菌。首先进行一项初步研究以确定提取物的最低抑菌浓度。冲洗组分为五个草药组和两个对照组。对牙齿进行冲洗后，使用qPCR测定剩余的微生物负荷。
采用单因素方差分析/克鲁斯卡尔 - 沃利斯检验，并进行事后Tukey's HSD检验，差异具有统计学意义（P < 0.05）。
结果表明，与其他提取物相比，印楝在减少根管内的粪肠球菌和白色念珠菌方面比5.25%的次氯酸钠更高效。
与5.25%的次氯酸钠相比，印楝叶提取物对粪肠球菌和白色念珠菌具有显著的抗菌效果。
