Cancer Nanotechnology Research Laboratory (CNRL), Faculty of Pharmacy, Alexandria University, Alexandria 21521, Egypt; Department of Industrial Pharmacy, Faculty of Pharmacy, Alexandria University, Alexandria 21521, Egypt.
Department of Pharmacy, School of Medicine, Faculty of Health, University of Tasmania, Hobart, Tasmania, Australia.
J Control Release. 2016 Dec 10;243:303-322. doi: 10.1016/j.jconrel.2016.10.023. Epub 2016 Oct 26.
Recently, a great interest has been paid to the development of hybrid protein-inorganic nanoparticles (NPs) for drug delivery and cancer diagnostics in order to combine the merits of both inorganic and protein nanocarriers. This review primarily discusses the most outstanding advances in the applications of the hybrids of naturally-occurring proteins with iron oxide, gadolinium, gold, silica, calcium phosphate NPs, carbon nanotubes, and quantum dots in drug delivery and cancer imaging. Various strategies that have been utilized for the preparation of protein-functionalized inorganic NPs and the mechanisms involved in the drug loading process are discussed. How can the protein functionalization overcome the limitations of colloidal stability, poor dispersibility and toxicity associated with inorganic NPs is also investigated. Moreover, issues relating to the influence of protein hybridization on the cellular uptake, tumor targeting efficiency, systemic circulation, mucosal penetration and skin permeation of inorganic NPs are highlighted. A special emphasis is devoted to the novel approaches utilizing the protein-inorganic nanohybrids in combined cancer therapy, tumor imaging, and theranostic applications as well as stimuli-responsive drug release from the nanohybrids.
最近，人们对开发用于药物输送和癌症诊断的蛋白质-无机杂化纳米粒子（NPs）产生了浓厚的兴趣，以结合无机和蛋白质纳米载体的优点。本综述主要讨论了将天然存在的蛋白质与氧化铁、钆、金、二氧化硅、磷酸钙 NPs、碳纳米管和量子点杂化在药物输送和癌症成像中的最新应用进展。讨论了用于制备蛋白质功能化无机 NPs 的各种策略以及涉及药物加载过程的机制。还研究了蛋白质官能化如何克服与无机 NPs 相关的胶体稳定性、分散性差和毒性的限制。此外，还强调了与蛋白质杂化对无机 NPs 的细胞摄取、肿瘤靶向效率、系统循环、黏膜穿透和皮肤渗透的影响有关的问题。特别强调了利用蛋白质-无机纳米杂化物在联合癌症治疗、肿瘤成像和治疗应用以及纳米杂化物中刺激响应性药物释放方面的新方法。
