Komaki Hisayuki, Ichikawa Natsuko, Oguchi Akio, Hamada Moriyuki, Harunari Enjuro, Kodani Shinya, Fujita Nobuyuki, Igarashi Yasuhiro
Biological Resource Center, National Institute of Technology and Evaluation (NBRC), Chiba, Japan.
NBRC, Tokyo, Japan.
Stand Genomic Sci. 2016 Oct 24;11:85. doi: 10.1186/s40793-016-0207-1. eCollection 2016.
sp. TP-A0867 (=NBRC 109436) produces structurally complex polyketides designated alchivemycins A and B. Here, we report the draft genome sequence of this strain together with features of the organism and assembly, annotation, and analysis of the genome sequence. The 9.9 Mb genome of sp. TP-A0867 encodes 8,385 putative ORFs, of which 7,232 were assigned with COG categories. We successfully identified a hybrid polyketide synthase (PKS)/ nonribosomal peptide synthetase (NRPS) gene cluster that could be responsible for alchivemycin biosynthesis, and propose the biosynthetic pathway. The alchivemycin biosynthetic gene cluster is also present in NRRL 5491, NBRC 16556, and NBRC 13981, which are taxonomically highly close to strain TP-A0867. This study shows a representative example that distribution of secondary metabolite genes is correlated with evolution within the genus .
菌株TP-A0867（=NBRC 109436）产生结构复杂的聚酮化合物，命名为阿奇霉素A和B。在此，我们报告该菌株的基因组草图序列，以及该生物体的特征、基因组序列的组装、注释和分析。菌株TP-A0867的9.9 Mb基因组编码8385个推定的开放阅读框（ORF），其中7232个被归类到COG类别。我们成功鉴定出一个可能负责阿奇霉素生物合成的混合聚酮合酶（PKS）/非核糖体肽合成酶（NRPS）基因簇，并提出了生物合成途径。阿奇霉素生物合成基因簇也存在于NRRL 5491、NBRC 16556和NBRC 13981中，它们在分类学上与菌株TP-A0867高度相近。这项研究展示了一个典型例子，即次生代谢物基因的分布与该属内的进化相关。
