Patel Dhairya H, Sharma Neeti
Symbiosis School of Biomedical Sciences, Symbiosis International University, Gram - Lavale, Taluka - Mulshi, Pune, India.
Onco Targets Ther. 2016 Oct 19;9:6445-6459. doi: 10.2147/OTT.S109253. eCollection 2016.
Considering the emerging concept of complementary and alternative medicine under the paucity of effective treatment for melanoma, we aimed to understand the effect of quercetin (Qu) on collagen I-induced epithelial-mesenchymal transition (EMT) in melanoma cells. To investigate the effect of Qu in melanoma cells, we used multiple methods, including real-time reverse transcription polymerase chain reaction, migration assay, and wound healing assay. We found that EMT was altered by Qu in melanoma cells. Qu-treated cells exhibited decreased migration and invasion activities. Mechanistically, a high expression of epithelial markers and a decrease in the expression of mesenchymal markers were found to be associated with reversal of EMT in melanoma cells. Time-dependent apoptosis was observed in Qu-treated melanoma cells, which was further confirmed by the upregulation in the protein levels of Caspase 3, a proapoptotic marker. Thus, our findings suggest Qu as a promising dietary compound under the new complementary and alternative medicine category of therapeutic drugs in the chemoprevention of melanoma.
考虑到在黑色素瘤有效治疗方法匮乏的情况下，补充和替代医学这一新兴概念，我们旨在了解槲皮素（Qu）对黑色素瘤细胞中I型胶原诱导的上皮-间质转化（EMT）的影响。为了研究Qu对黑色素瘤细胞的作用，我们使用了多种方法，包括实时逆转录聚合酶链反应、迁移试验和伤口愈合试验。我们发现Qu改变了黑色素瘤细胞中的EMT。用Qu处理的细胞表现出迁移和侵袭活性降低。从机制上讲，上皮标志物的高表达和间质标志物表达的降低与黑色素瘤细胞中EMT的逆转有关。在用Qu处理的黑色素瘤细胞中观察到时间依赖性凋亡，这通过促凋亡标志物Caspase 3蛋白水平的上调得到进一步证实。因此，我们的研究结果表明，在新的补充和替代医学类治疗药物中，Qu作为一种有前景的膳食化合物可用于黑色素瘤的化学预防。
