Sharmin Ani Nushrat, Chakraborty Sudip, Moniruzzaman Md
Department of Pharmacy, Stamford University Bangladesh, 51 Shiddheswari Road, Dhaka 1217, Bangladesh.
Adv Pharmacol Sci. 2016;2016:3790860. doi: 10.1155/2016/3790860. Epub 2016 Oct 12.
L. is a perennial shrub, belonging to the family Rutaceae. Traditionally, the leaves of this plant are extensively used in treatment of a wide range of diseases and disorders including pain and inflammation. Although researchers have revealed the antinociceptive effects of this plant's leaves during past few years, the mechanisms underlying these effects are still unknown. Therefore, the present study evaluated some antinociceptive mechanisms of the methanolic extract of (MEMK) leaves along with its antinociceptive potential using several animal models. The antinociceptive effects of MEMK were evaluated using formalin-induced licking and acetic acid-induced writhing tests at the doses of 50, 100, and 200 mg/kg. In addition, we also justified the possible participations of glutamatergic system and ATP-sensitive potassium channels in the observed activities. Our results demonstrated that MEMK significantly ( < 0.01) inhibited the pain thresholds induced by formalin and acetic acid in a dose-dependent manner. MEMK also significantly ( < 0.01) suppressed glutamate-induced pain. Moreover, pretreatment with glibenclamide (an ATP-sensitive potassium channel blocker) at 10 mg/kg significantly ( < 0.05) reversed the MEMK-mediated antinociception. These revealed that MEMK might have the potential to interact with glutamatergic system and the ATP-sensitive potassium channels to exhibit its antinociceptive activities. Therefore, our results strongly support the antinociceptive effects of leaves and provide scientific basis of their analgesic uses in the traditional medicine.
L.是一种多年生灌木，属于芸香科。传统上，这种植物的叶子被广泛用于治疗多种疾病和紊乱，包括疼痛和炎症。尽管研究人员在过去几年中揭示了这种植物叶子的抗伤害感受作用，但其作用机制仍然未知。因此，本研究使用多种动物模型评估了L.叶子甲醇提取物（MEMK）的一些抗伤害感受机制及其抗伤害感受潜力。在50、100和200mg/kg剂量下，使用福尔马林诱导的舔舐和醋酸诱导的扭体试验评估MEMK的抗伤害感受作用。此外，我们还验证了谷氨酸能系统和ATP敏感性钾通道在观察到的活性中可能的参与情况。我们的结果表明，MEMK以剂量依赖性方式显著（P<0.01）抑制福尔马林和醋酸诱导的疼痛阈值。MEMK还显著（P<0.01）抑制谷氨酸诱导的疼痛。此外，用10mg/kg格列本脲（一种ATP敏感性钾通道阻滞剂）预处理显著（P<0.05）逆转了MEMK介导的抗伤害感受作用。这些结果表明，MEMK可能有潜力与谷氨酸能系统和ATP敏感性钾通道相互作用以发挥其抗伤害感受活性。因此，我们的结果有力地支持了L.叶子的抗伤害感受作用，并为其在传统医学中的镇痛用途提供了科学依据。
