Department of Pharmacology, MGV's Pharmacy College, Panchavati, Nasik, Maharashtra, India.
Indian J Pharmacol. 2012 Jan;44(1):15-9. doi: 10.4103/0253-7613.91860.
To evaluate the antinociceptive activity of acute and chronic administration of petroleum ether extract of Murraya koenigii L. leaves (PMK) and total alkaloids separated from petroleum ether extract of Murraya koenigii leaves (AMK) in mice.
PMK was subjected for isolation of total alkaloid fraction AMK. The antinociceptive activity of PMK (100 and 300 mg/kg, p.o.) and AMK (100 and 300 mg/kg, p.o.), after acute and chronic administration (for 15 days), was evaluated using peripheral model like acetic acid-induced writhing method and central model like hot plate method and tail immersion method. Statistical analysis was carried out by one-way ANOVA followed by Dunnett's test.
In acute studies, PMK and AMK significantly and dose-dependently reduced the number of acetic acid-induced writhing, significantly increased the latency of paw licking in hot plate method, and significantly increased the basal reaction time in tail immersion method. With chronic administration of PMK and AMK, highest activity was observed on day 9 in acetic acid-induced writhing model. In hot plate and tail immersion method, chronic administration of PMK and AMK initially showed fluctuating responses but produced highest degree of antinociception on day 9 of the study.
The degree of antinociception produced by PMK and AMK at the end of 15 days study suggest that Murraya koenigii has potential to use as an analgesic.
评价急性和慢性给予九里香叶石油醚提取物（PMK）和从九里香叶石油醚提取物中分离得到的总生物碱（AMK）对小鼠的镇痛活性。
PMK 用于分离总生物碱部分 AMK。通过外周模型（醋酸诱导扭体法）和中枢模型（热板法和尾浸法）评估 PMK（100 和 300mg/kg，po）和 AMK（100 和 300mg/kg，po）在急性和慢性给药（15 天）后的镇痛活性。统计分析采用单因素方差分析，然后进行 Dunnett 检验。
在急性研究中，PMK 和 AMK 显著且剂量依赖性地减少了醋酸诱导的扭体次数，显著增加了热板法中舔爪的潜伏期，显著增加了尾浸法中的基础反应时间。在慢性给予 PMK 和 AMK 的研究中，在醋酸诱导的扭体模型中，第 9 天观察到最高的活性。在热板和尾浸法中，PMK 和 AMK 的慢性给药最初表现出波动的反应，但在研究的第 9 天产生了最高程度的镇痛作用。
在 15 天研究结束时，PMK 和 AMK 产生的镇痛程度表明九里香具有作为镇痛药的潜力。
