Joubert Lydia-Marie, Ferreira Jose Ag, Stevens David A, Nazik Hasan, Cegelski Lynette
Cell Sciences Imaging Facility, Stanford University School of Medicine, Stanford, CA, USA.
Division of Infectious Diseases and Geographic Medicine, Stanford University, Stanford, CA, USA; California Institute for Medical Research, San Jose, CA, USA.
J Microbiol Methods. 2017 Jan;132:46-55. doi: 10.1016/j.mimet.2016.11.002. Epub 2016 Nov 9.
Aspergillus fumigatus biofilms consist of a three-dimensional network of cellular hyphae and extracellular matrix. They are involved in infections of immune-compromised individuals, particularly those with cystic fibrosis. These structures are associated with persistence of infection, resistance to host immunity, and antimicrobial resistance. Thorough understanding of structure and function is imperative in the design of therapeutic drugs. Optimization of processing parameters, including aldehyde fixation, heavy metal contrasting, drying techniques and Ionic Liquid treatment, was undertaken for an ultrastructural approach to understand cellular and extracellular biofilm components. Conventional and Variable Pressure Scanning Electron Microscopy were applied to analyze the structure of biofilms attached to plastic and formed at an air-liquid interface.
烟曲霉生物膜由细胞菌丝和细胞外基质的三维网络组成。它们与免疫功能低下个体的感染有关，尤其是囊性纤维化患者。这些结构与感染的持续存在、对宿主免疫的抵抗以及抗菌耐药性有关。在治疗药物设计中，彻底了解其结构和功能至关重要。为了采用超微结构方法了解细胞和细胞外生物膜成分，对包括醛固定、重金属对比、干燥技术和离子液体处理在内的处理参数进行了优化。应用常规和可变压力扫描电子显微镜分析附着在塑料上并在气液界面形成的生物膜的结构。
