Patel Dipak, Desai Swati, Devkar Ranjitsinh, Ramachandran A V
Division of Phytotherapeutics and Metabolic Endocrinology, Department of Zoology, Faculty of Science, The M. S. University of Baroda, Gujarat, India.
EXCLI J. 2012 Aug 28;11:566-575. eCollection 2012.
L. (CS) seeds are known to possess therapeutic potentials against a variety of physiological disorders. This study assesses acute and sub-chronic toxicity profile of hydro-methanolic extract of CS seeds using OECD guidelines. In acute toxicity study, mice were once orally administered 1000, 3000 and 5000 mg/kg body weight of CS extract. There were no any behavioral alterations or mortality recorded in CS treated groups. The LD value was more than 5000 mg/kg body weight. In the sub-chronic oral toxicity study, the animals were orally administered with CS extract (1000, 2000 and 3000mg/kg body weight) daily for 28 days whereas; vehicle control group received 0.5 % carboxy methyl cellulose. There was significant reduction in food intake, body weight gain and plasma lipid profiles of CS and CS (2000 and 3000 mg/kg body weight respectively) groups as compared to the control group. However, there were no alterations in haematological profile, relative organ weights, histology and plasma markers of damage of vital organs (heart, liver and kidney). The overall finding of this study indicates that CS extract is non-toxic up to 3000 mg/kg body weight and can be considered as safe for consumption.
已知L.（CS）种子具有针对多种生理紊乱的治疗潜力。本研究使用经合组织指南评估CS种子水甲醇提取物的急性和亚慢性毒性特征。在急性毒性研究中，小鼠一次性口服给予1000、3000和5000 mg/kg体重的CS提取物。CS处理组未记录到任何行为改变或死亡情况。LD值大于5000 mg/kg体重。在亚慢性口服毒性研究中，动物每天口服给予CS提取物（1000、2000和3000 mg/kg体重），持续28天；而溶剂对照组接受0.5%羧甲基纤维素。与对照组相比，CS组和CS（分别为2000和3000 mg/kg体重）组的食物摄入量、体重增加和血浆脂质谱均显著降低。然而，血液学指标、相对器官重量、组织学以及重要器官（心脏、肝脏和肾脏）损伤的血浆标志物均无变化。本研究的总体结果表明，CS提取物在高达3000 mg/kg体重时无毒，可被认为食用安全。
