Tan T H, Horikoshi M, Roeder R G
Laboratory of Biochemistry and Molecular Biology, Rockefeller University, New York, New York 10021.
Mol Cell Biol. 1989 Apr;9(4):1733-45. doi: 10.1128/mcb.9.4.1733-1745.1989.
Within the human T-cell leukemia virus type I promoter, there are three copies of a 21-base-pair repeat (hereafter called the tax-responsive element [TRE]) that both contributes to basal promoter activity and mediates induction by the viral activator TAX. We have identified and separated three nuclear proteins that interact with the TRE. The TRE-binding protein designated TREB-3 bound more avidly to a multimerized TRE than to a single-copy TRE, while the other two TRE-binding proteins, TREB-1 and TREB-2, bound equally well to either TRE. TREB-1 has been purified to near homogeneity, and binding activity was localized to a protein of 35 to 43 kilodaltons. The affinity-purified TREB-1 activated transcription from the human T-cell leukemia virus type I promoter in vitro. The purified TREB-1 fraction contained activating transcription factor binding activity and showed a cooperative interaction with the TATA-binding factor (TFIID) on the adenovirus E4 promoter.
在人类I型T细胞白血病病毒启动子内，存在一个21个碱基对重复序列的三个拷贝（以下称为tax反应元件 [TRE]），它既有助于基础启动子活性，又介导病毒激活因子TAX的诱导作用。我们已经鉴定并分离出三种与TRE相互作用的核蛋白。命名为TREB - 3的TRE结合蛋白与多聚化的TRE结合比与单拷贝TRE结合更紧密，而其他两种TRE结合蛋白TREB - 1和TREB - 2与两种TRE的结合效果相同。TREB - 1已被纯化至近乎均一，其结合活性定位于一种35至43千道尔顿的蛋白质。亲和纯化的TREB - 1在体外可激活人类I型T细胞白血病病毒启动子的转录。纯化的TREB - 1组分具有激活转录因子结合活性，并在腺病毒E4启动子上与TATA结合因子（TFIID）表现出协同相互作用。
