在猫中使用多重检测法比较血清和血浆细胞因子值。
A comparison of serum and plasma cytokine values using a multiplexed assay in cats.
作者信息
Gruen Margaret E, Messenger Kristen M, Thomson Andrea E, Griffith Emily H, Paradise Hayley, Vaden Shelly, Lascelles B D X
机构信息
Comparative Pain Research Program, Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, 1060 William Moore Drive, Raleigh, NC 27607, USA; Comparative Medicine Institute, North Carolina State University, Raleigh, NC 27695, USA.
Comparative Medicine Institute, North Carolina State University, Raleigh, NC 27695, USA; Department of Molecular and Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, 1060 William Moore Drive, Raleigh, NC, 27607, Raleigh, NC, USA.
出版信息
Vet Immunol Immunopathol. 2016 Dec;182:69-73. doi: 10.1016/j.vetimm.2016.10.003. Epub 2016 Oct 14.
BACKGROUND
Degenerative joint disease (DJD) is highly prevalent in cats, and pain contributes to morbidity. In humans, alterations of cytokine concentrations have been associated with joint deterioration and pain. Similar changes have not been investigated in cats. Cytokine concentrations can be measured using multiplex technology with small samples of serum or plasma, however, serum and plasma are not interchangeable for most bioassays. Correlations for cytokine concentrations between serum and plasma have not been evaluated in cats.
OBJECTIVE
To evaluate the levels of detection and agreement between serum and plasma samples in cats.
ANIMALS
Paired serum and plasma samples obtained from 38 cats.
METHODS
Blood was collected into anti-coagulant free and EDTA Vacutainer tubes, serum or plasma extracted, and samples frozen at -80°C until testing. Duplicate samples were tested using a 19-plex feline cytokine/chemokine magnetic bead panel.
RESULTS
Agreement between serum and plasma for many analytes was high, however correlation coefficients ranged from -0.01 to 0.97. Results from >50% of samples were below the lower limit of quantification for both serum and plasma for nine analytes, and for an additional three analytes for plasma only.
CONCLUSIONS AND CLINICAL IMPORTANCE
While serum and plasma agreement was generally good, detection was improved using serum samples.
背景
退行性关节病（DJD）在猫中非常普遍，疼痛会导致发病。在人类中，细胞因子浓度的改变与关节退变和疼痛有关。猫中尚未对类似变化进行研究。细胞因子浓度可以使用多重技术通过少量血清或血浆样本进行测量，然而，对于大多数生物测定，血清和血浆不可互换。猫血清和血浆之间细胞因子浓度的相关性尚未评估。
目的
评估猫血清和血浆样本之间的检测水平和一致性。
动物
从38只猫获得配对的血清和血浆样本。
方法
将血液采集到不含抗凝剂的管和乙二胺四乙酸（EDTA）真空采血管中，提取血清或血浆，并将样本在-80°C下冷冻直至检测。使用19种猫细胞因子/趋化因子磁珠板对重复样本进行检测。
结果
许多分析物的血清和血浆之间的一致性很高，然而相关系数范围为-0.01至0.97。超过50%的样本结果低于9种分析物的血清和血浆定量下限，另外3种分析物仅血浆低于定量下限。
结论和临床意义
虽然血清和血浆的一致性总体良好，但使用血清样本检测效果更好。
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