Foster J D, Sample S, Kohler R, Watson K, Muir P, Trepanier L A
Department of Medical Sciences, University of Wisconsin-Madison, Madison, WI.
J Vet Intern Med. 2014 May-Jun;28(3):905-11. doi: 10.1111/jvim.12351. Epub 2014 Apr 3.
Immune-mediated polyarthopathy (IMPA) is common in dogs, and is monitored by serial arthrocenteses.
HYPOTHESIS/OBJECTIVES: Plasma C-reactive protein (CRP), interleukin-6 (IL-6), and CXCL8 (interleukin-8) would serve as noninvasive markers of joint inflammation in IMPA.
Nine client-owned dogs with idiopathic IMPA; 6 healthy controls.
Prospective study. Plasma CRP, IL-6, and CXCL8 were measured by ELISA at baseline, 2, and 4 weeks during treatment with prednisone at 50 mg/m(2) /day. Arthrocenteses, the canine brief pain inventory (CBPI), and accelerometry collars were used to assess joint inflammation, lameness, and mobility at all 3 time points.
C-reactive protein concentrations were higher in IMPA dogs (median 91.1 μg/mL, range 76.7-195.0) compared with controls (median <6.3 μg/mL, <6.3-13.7; P = .0035), and were significantly lower at week 2 (10.6 μg/mL, <6.3-48.8) and week 4 (<6.3 μg/mL, <6.3-24.4; P < .001). C-reactive protein was correlated with median CBPI scores (r = 0.68; P = .0004), joint cellularity (r = 0.49, P = .011), and mobility by accelerometry (r = -0.42, P = .048). Plasma IL-6 concentrations were also higher in IMPA dogs (median 45.9 pg/mL), compared with controls (median <15.7 pg/mL; P = .0008). IL-6 was lower in IMPA dogs by week 4 (<15.7 pg/mL; P = .0099), and was modestly correlated with CBPI scores (r = 0.47, P = .023). CXCL8 did not differ significantly between IMPA and healthy dogs.
Plasma CRP and IL-6 might be useful surrogate markers of synovial inflammation and disease activity in dogs with IMPA.
免疫介导性多关节炎（IMPA）在犬类中很常见，通过连续关节穿刺术进行监测。
假设/目的：血浆C反应蛋白（CRP）、白细胞介素-6（IL-6）和CXCL8（白细胞介素-8）可作为IMPA关节炎症的非侵入性标志物。
9只患有特发性IMPA的客户拥有犬；6只健康对照犬。
前瞻性研究。在以50mg/m²/天的泼尼松治疗期间，于基线、第2周和第4周通过ELISA法测定血浆CRP、IL-6和CXCL8。在所有3个时间点使用关节穿刺术、犬简短疼痛量表（CBPI）和加速度计项圈来评估关节炎症、跛行和活动能力。
与对照组（中位数<6.3μg/mL，范围<6.3 - 13.7）相比，IMPA犬的C反应蛋白浓度更高（中位数91.1μg/mL，范围76.7 - 195.0；P = 0.0035），且在第2周（10.6μg/mL，<6.3 - 48.8）和第4周（<6.3μg/mL，<6.3 - 24.4；P < 0.001）时显著降低。C反应蛋白与CBPI中位数评分相关（r = 0.68；P = 0.0004）、与关节细胞密度相关（r = 0.49，P = 0.011）以及与加速度计测量的活动能力相关（r = -0.42，P = 0.048）。与对照组（中位数<15.7pg/mL）相比，IMPA犬的血浆IL-6浓度也更高（中位数45.9pg/mL；P = 0.0008）。到第4周时，IMPA犬的IL-6降低（<15.7pg/mL；P = 0.0099），并且与CBPI评分有适度相关性（r = 0.47，P = 0.023）。IMPA犬和健康犬之间的CXCL8没有显著差异。
血浆CRP和IL-6可能是IMPA犬滑膜炎症和疾病活动的有用替代标志物。
