Vu-Van Tu, Pham-Duc Phuc, Winkler Mirko S, Zurbrügg Christian, Zinsstag Jakob, Le Thi Thanh Huong, Bich Tran Huu, Nguyen-Viet Hung
Center for Public Health and Ecosystem Research (CENPHER), Hanoi University of Public Health, Hanoi, Vietnam.
Hoa Binh Provincial General Hospital, Hoa Binh, Vietnam.
Int J Public Health. 2017 Feb;62(Suppl 1):103-111. doi: 10.1007/s00038-016-0920-y. Epub 2016 Nov 22.
We studied the influence of different additive materials (lime, and rice husk) and aeration conditions on Ascaris lumbricoides egg die-off in 24 vaults of an experimental excreta storage unit.
Excreta samples were collected once every two weeks over a 181-day period. Temperature, pH, and moisture content were recorded. A. lumbricoides eggs were quantitatively analyzed by the Romanenko method, which identified and counted live and dead eggs.
From the first sampling (0 storage day) to the final sampling (181 storage days) the average percentage of viable A. lumbricoides eggs decreased gradually from 76.72 ± 11.23% (mean ± SD) to 8.26 ± 5.20%. The storage time and the high pH value significantly increased the die-off of helminth eggs. Over 181 storage days, all vaults option effectively reduced A. lumbricoides eggs die-off.
The best vault option, with aeration and 10% lime per total weight, met the WHO standard for excreta treatment on the 111th storage day.
我们研究了不同添加材料（石灰和稻壳）及曝气条件对实验性排泄物储存单元24个拱顶中蛔虫卵死亡情况的影响。
在181天的时间里，每两周采集一次排泄物样本。记录温度、pH值和水分含量。采用罗曼年科法对蛔虫卵进行定量分析，该方法可识别并计数活卵和死卵。
从首次采样（储存0天）到最后采样（储存181天），活蛔虫卵的平均百分比从76.72 ± 11.23%（平均值±标准差）逐渐降至8.26 ± 5.20%。储存时间和高pH值显著增加了蠕虫卵的死亡。在181天的储存期内，所有拱顶方案均有效降低了蛔虫卵的死亡。
最佳拱顶方案，即曝气且每总重量含10%石灰，在储存第111天时达到了世界卫生组织的排泄物处理标准。
