Sierakowska H, Williams K R, Szer I S, Szer W
Department of Biochemistry, New York University School of Medicine 10016.
Clin Exp Immunol. 1993 Dec;94(3):435-9. doi: 10.1111/j.1365-2249.1993.tb08214.x.
The 45-kD autoantigen associated with juvenile rheumatoid arthritis (JRA) has been isolated from HeLa cell nuclei and purified about 2500-fold to near homogeneity in a five-step chromatographic procedure. Purification of the antigen was monitored by immunoblot assays using a nearly monospecific anti-45-kD serum from a child with JRA. Tryptic peptide mapping and partial amino acid sequencing of the purified 45-kD antigen demonstrated its identity with the DEK protein. DEK is a 43-kD protein of unknown function expressed by the putative oncogene dek located on chromosome 6. As a result of a (6;9) translocation offociated with a rare subtype of acute myeloid leukaemia a chimeric protein containing most of DEK amino acids at the N-terminus is found in leukaemic cells (von Linden et al., Mol Cell Biol. 1992; 12: 1687-97). The 43-kD DEK was detected by immunoblotting with serum from a patient with JRA in a variety of rat tissues, and was most abundant in the spleen and in bone marrow.
与青少年类风湿性关节炎（JRA）相关的45-kD自身抗原已从HeLa细胞核中分离出来，并通过五步色谱法纯化了约2500倍，达到近乎纯一的状态。使用来自一名JRA患儿的近乎单特异性的抗45-kD血清，通过免疫印迹分析监测抗原的纯化过程。对纯化的45-kD抗原进行胰蛋白酶肽图谱分析和部分氨基酸测序，结果表明它与DEK蛋白相同。DEK是一种43-kD的蛋白质，功能未知，由位于6号染色体上的假定癌基因dek表达。由于与一种罕见的急性髓性白血病亚型相关的（6；9）易位，在白血病细胞中发现了一种在N端含有大部分DEK氨基酸的嵌合蛋白（冯·林登等人，《分子细胞生物学》。1992年；12：1687 - 97）。用JRA患者的血清通过免疫印迹法在多种大鼠组织中检测到了43-kD的DEK，且在脾脏和骨髓中含量最为丰富。
