• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

培养的里德-斯腾伯格细胞系HDLM-1、HDLM-1d和KM-H2中白细胞介素1产生的异质性。

Heterogeneity of interleukin 1 production in cultured Reed-Sternberg cell lines HDLM-1, HDLM-1d, and KM-H2.

作者信息

Hsu S M, Krupen K, Lachman L B

机构信息

Department of Pathology, University of Texas Health Science Center, Houston.

出版信息

Am J Pathol. 1989 Jul;135(1):33-8.

PMID:2789003
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1880234/
Abstract

Interleukin 1 (IL-1) is known for its role in modulating the immune response and is required for initiation of lymphocyte proliferation by means of increased IL-2 production by lymphocytes. Previously, the expression of IL-1 in H-RS cells in tissue sections was shown by using immunoperoxidase staining. For further confirmation, the production of IL-1 in cultured cells of the H-RS cell lines HDLM-1, HDLM-1d, and KM-H2 was examined by using a murine D10.G4.1 T cell proliferation bioassay and Northern blot hybridization with specific IL-1 cDNA probes. It was confirmed that two types of H-RS cells, HDLM-1 and KM-H2, can secrete IL-1, especially after treatment with phorbol ester. The amounts of IL-1 in H-RS cell culture medium ranged from approximately 0.5 to 2.5 ng/ml. The major IL-1 secreted by HDLM-1 cells was IL-1 alpha, and by KM-H2 cells was IL-1 beta. HDLM-1d cells did not produce IL-1. This finding indicates the heterogeneity of IL-1 production in H-RS cells. Such heterogeneity may apply to H-RS cells in vivo, based on the variable IL-1 staining of these cells in lymphoid tissues.

摘要

白细胞介素1（IL-1）以其在调节免疫反应中的作用而闻名，并且淋巴细胞通过增加IL-2的产生来启动增殖需要IL-1。以前，通过免疫过氧化物酶染色显示了组织切片中H-RS细胞中IL-1的表达。为了进一步证实，使用鼠D10.G4.1 T细胞增殖生物测定法和与特异性IL-1 cDNA探针的Northern印迹杂交，检测了H-RS细胞系HDLM-1、HDLM-1d和KM-H2的培养细胞中IL-1的产生。已证实两种类型的H-RS细胞，即HDLM-1和KM-H2，能够分泌IL-1，尤其是在用佛波酯处理后。H-RS细胞培养基中IL-1的量约为0.5至2.5 ng/ml。HDLM-1细胞分泌的主要IL-1是IL-1α，而KM-H2细胞分泌的是IL-1β。HDLM-1d细胞不产生IL-1。这一发现表明H-RS细胞中IL-1产生的异质性。基于这些细胞在淋巴组织中可变的IL-1染色，这种异质性可能也适用于体内的H-RS细胞。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a0f/1880234/0bc1d6727123/amjpathol00115-0037-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a0f/1880234/0bc1d6727123/amjpathol00115-0037-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a0f/1880234/0bc1d6727123/amjpathol00115-0037-a.jpg

相似文献

1
Heterogeneity of interleukin 1 production in cultured Reed-Sternberg cell lines HDLM-1, HDLM-1d, and KM-H2.培养的里德-斯腾伯格细胞系HDLM-1、HDLM-1d和KM-H2中白细胞介素1产生的异质性。
Am J Pathol. 1989 Jul;135(1):33-8.
2
Production of tumor necrosis factor-alpha and lymphotoxin by cells of Hodgkin's neoplastic cell lines HDLM-1 and KM-H2.霍奇金氏肿瘤细胞系HDLM-1和KM-H2的细胞产生肿瘤坏死因子-α和淋巴毒素。
Am J Pathol. 1989 Oct;135(4):735-45.
3
Expression of p55 (Tac) interleukin-2 receptor (IL-2R), but not p75 IL-2R, in cultured H-RS cells and H-RS cells in tissues.培养的霍奇金-里德-斯腾伯格（H-RS）细胞及组织中的H-RS细胞表达p55（Tac）白细胞介素-2受体（IL-2R），但不表达p75 IL-2R。
Am J Pathol. 1990 Apr;136(4):735-44.
4
Abundant expression of transforming growth factor-beta 1 and -beta 2 by Hodgkin's Reed-Sternberg cells and by reactive T lymphocytes in Hodgkin's disease.霍奇金淋巴瘤中的霍奇金-里德-斯腾伯格细胞和反应性T淋巴细胞大量表达转化生长因子-β1和-β2 。
Hum Pathol. 1993 Mar;24(3):249-55. doi: 10.1016/0046-8177(93)90034-e.
5
Reed-Sternberg cells in Hodgkin's cell lines HDLM, L-428, and KM-H2 are not actively replicating: lack of bromodeoxyuridine uptake by multinuclear cells in culture.霍奇金细胞系HDLM、L-428和KM-H2中的里德-斯腾伯格细胞并非在活跃复制：培养中的多核细胞缺乏溴脱氧尿苷摄取。
Blood. 1988 May;71(5):1382-9.
6
Expression of macrophage colony-stimulating factor (M-CSF) in two Hodgkin's Reed-Sternberg (H-RS) cell lines, HDLM-1 and KM-H2, and in H-RS cells in tissues.巨噬细胞集落刺激因子（M-CSF）在两种霍奇金里德-斯腾伯格（H-RS）细胞系HDLM-1和KM-H2以及组织中的H-RS细胞中的表达。
Int J Hematol. 1991 Aug;54(4):315-26.
7
Lack of effect of colony-stimulating factors, interleukins, interferons, and tumor necrosis factor on the growth and differentiation of cultured Reed-Sternberg cells. Comparison with effects of phorbol ester and retinoic acid.集落刺激因子、白细胞介素、干扰素及肿瘤坏死因子对培养的里德-斯腾伯格细胞生长和分化无作用。与佛波酯和视黄酸作用的比较。
Am J Pathol. 1990 Jan;136(1):181-9.
8
Lymphocyte functional antigens stabilize agglutination between Reed-Sternberg cells and T cells, but are not responsible for homotypic binding of Hodgkin's Reed-Sternberg cells.淋巴细胞功能抗原可稳定里德-施特恩贝格细胞与T细胞之间的凝集，但并非霍奇金里德-施特恩贝格细胞同型结合的原因。
Am J Pathol. 1990 Sep;137(3):563-74.
9
Expression of cytokine genes, cytokine receptor genes, and transcription factors in cultured Hodgkin and Reed-Sternberg cells.培养的霍奇金和里德-斯腾伯格细胞中细胞因子基因、细胞因子受体基因及转录因子的表达
Cancer Res. 1992 Jun 15;52(12):3353-60.
10
Functional coexpression of Interleukin (IL)-7 and its receptor (IL-7R) on Hodgkin and Reed-Sternberg cells: Involvement of IL-7 in tumor cell growth and microenvironmental interactions of Hodgkin's lymphoma.白细胞介素（IL）-7及其受体（IL-7R）在霍奇金和里德-斯腾伯格细胞上的功能性共表达：IL-7参与霍奇金淋巴瘤的肿瘤细胞生长和微环境相互作用。
Int J Cancer. 2009 Sep 1;125(5):1092-101. doi: 10.1002/ijc.24389.

引用本文的文献

1
Cultured Reed-Sternberg cells HDLM-1 and KM-H2 can be induced to become histiocytelike cells. H-RS cells are not derived from lymphocytes.培养的里德-斯腾伯格细胞HDLM-1和KM-H2可被诱导成为组织细胞样细胞。霍奇金里德-斯腾伯格细胞并非来源于淋巴细胞。
Am J Pathol. 1990 Aug;137(2):353-67.
2
Cytokine expression in T-cell lymphomas and Hodgkin's disease. Its possible implication in autocrine or paracrine production as a potential basis for neoplastic growth.细胞因子在T细胞淋巴瘤和霍奇金病中的表达。其在自分泌或旁分泌产生中的可能意义，作为肿瘤生长的潜在基础。
Am J Pathol. 1991 Nov;139(5):1173-80.
3
Cytokine production (IL-1 alpha, IL-1 beta, and TNF alpha) and endothelial cell activation (ELAM-1 and HLA-DR) in reactive lymphadenitis, Hodgkin's disease, and in non-Hodgkin's lymphomas. An immunocytochemical study.

本文引用的文献

1
Leu M1 and peanut agglutinin stain the neoplastic cells of Hodgkin's disease.白细胞共同抗原M1和花生凝集素可使霍奇金病的肿瘤细胞着色。
Am J Clin Pathol. 1984 Jul;82(1):29-32. doi: 10.1093/ajcp/82.1.29.
2
Production of colony-stimulating factors by Hodgkin cell lines.霍奇金细胞系产生集落刺激因子。
Int J Cancer. 1983 Mar 15;31(3):269-74. doi: 10.1002/ijc.2910310303.
3
Induction of receptors for interleukin 2 requires T cell Ag:Ia receptor crosslinking and interleukin 1.白细胞介素2受体的诱导需要T细胞抗原：Ia受体交联和白细胞介素1。
反应性淋巴结炎、霍奇金病及非霍奇金淋巴瘤中细胞因子产生（白细胞介素-1α、白细胞介素-1β和肿瘤坏死因子α）及内皮细胞活化（E-选择素和人类白细胞抗原-DR）：一项免疫细胞化学研究
Am J Pathol. 1990 Nov;137(5):1163-71.
4
Expression of p55 (Tac) interleukin-2 receptor (IL-2R), but not p75 IL-2R, in cultured H-RS cells and H-RS cells in tissues.培养的霍奇金-里德-斯腾伯格（H-RS）细胞及组织中的H-RS细胞表达p55（Tac）白细胞介素-2受体（IL-2R），但不表达p75 IL-2R。
Am J Pathol. 1990 Apr;136(4):735-44.
5
Lack of effect of colony-stimulating factors, interleukins, interferons, and tumor necrosis factor on the growth and differentiation of cultured Reed-Sternberg cells. Comparison with effects of phorbol ester and retinoic acid.集落刺激因子、白细胞介素、干扰素及肿瘤坏死因子对培养的里德-斯腾伯格细胞生长和分化无作用。与佛波酯和视黄酸作用的比较。
Am J Pathol. 1990 Jan;136(1):181-9.
6
Lymphomas of true histiocytic origin. Expression of different phenotypes in so-called true histiocytic lymphoma and malignant histiocytosis.真正组织细胞源性淋巴瘤。所谓真正组织细胞淋巴瘤和恶性组织细胞增多症中不同表型的表达。
Am J Pathol. 1991 Jun;138(6):1389-404.
7
Interleukin-6, but not interleukin-4, is expressed by Reed-Sternberg cells in Hodgkin's disease with or without histologic features of Castleman's disease.在伴有或不伴有卡斯特曼病组织学特征的霍奇金病中，里德-施特恩伯格细胞表达白细胞介素-6，而非白细胞介素-4。
Am J Pathol. 1992 Jul;141(1):129-38.
8
Expression and cell distribution of the intercellular adhesion molecule, vascular cell adhesion molecule, endothelial leukocyte adhesion molecule, and endothelial cell adhesion molecule (CD31) in reactive human lymph nodes and in Hodgkin's disease.细胞间黏附分子、血管细胞黏附分子、内皮白细胞黏附分子及内皮细胞黏附分子（CD31）在反应性人类淋巴结和霍奇金病中的表达及细胞分布
Am J Pathol. 1992 Jun;140(6):1337-44.
Lymphokine Res. 1984 Summer;3(4):175-82.
4
Role of arachidonate metabolism in the immunoregulatory function of human leukocytic pyrogen/lymphocyte-activating factor/interleukin 1.花生四烯酸代谢在人白细胞致热原/淋巴细胞激活因子/白细胞介素1免疫调节功能中的作用。
J Immunol. 1983 Feb;130(2):890-5.
5
Potentiation of fibroblast growth by nodular sclerosing Hodgkin's disease cell cultures.结节性硬化型霍奇金病细胞培养对成纤维细胞生长的增强作用。
Blood. 1982 Jul;60(1):228-37.
6
Implications of persistent T cell abnormalities for the etiology of Hodgkin's disease.持续性T细胞异常对霍奇金淋巴瘤病因学的影响。
Cancer Treat Rep. 1982 Apr;66(4):681-7.
7
Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.变性RNA与转移至硝酸纤维素膜上的小DNA片段的杂交。
Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5. doi: 10.1073/pnas.77.9.5201.
8
Phenotypic expression of Hodgkin's and Reed-Sternberg cells in Hodgkin's disease.霍奇金病中霍奇金和里德-斯腾伯格细胞的表型表达。
Am J Pathol. 1985 Feb;118(2):209-17.
9
Identification of a membrane-associated interleukin 1 in macrophages.巨噬细胞中一种膜相关白细胞介素1的鉴定。
Proc Natl Acad Sci U S A. 1985 Feb;82(4):1204-8. doi: 10.1073/pnas.82.4.1204.
10
Human monocyte heterogeneity: interleukin 1 and prostaglandin E2 production by separate subsets.人类单核细胞异质性：不同亚群产生白细胞介素-1和前列腺素E2
Eur J Immunol. 1985 Jan;15(1):48-51. doi: 10.1002/eji.1830150110.