Jiu Yueguang, Zhu Shun, Khan Sher Bahadar, Sun Mengzhen, Zou Geng, Meng Xianrong, Wu Bin, Zhou Rui, Li Shaowen
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University , Wuhan, People's Republic of China .
Microb Drug Resist. 2017 Jul;23(5):651-659. doi: 10.1089/mdr.2016.0132. Epub 2016 Nov 28.
The antimicrobial resistance of Salmonella strains is rapidly increasing worldwide, which poses significant threats to animal and public health. In this study, a total of 249 porcine Salmonella isolates collected in China during 2008-2015 were examined, including 155 clinical isolates from diseased pigs and 94 nonclinical isolates from healthy pigs. Based on the minimum inhibitory concentration of seven antimicrobial agents, 96.4% of the isolates were resistant to at least one of the tested antibiotics and 81.0% of them showed multidrug resistance. The highest antimicrobial resistance was observed for tetracycline (85.9%), and the lowest was found for cefotaxime (13.3%). The isolates from diseased pigs exhibited significantly higher levels of antimicrobial resistance than those from healthy pigs. Twenty-two isolates from healthy pigs were resistant to ciprofloxacin, which may inhibit the curative effectiveness of fluoroquinolones on bacterial food-borne poisoning and infections in humans caused by contaminated food. Moreover, cefotaxime resistance of the strains isolated from diseased pigs during 2013-2015 was significantly higher compared with the strains isolated during 2008-2010. Further study showed that the correlation between phenotypic and genotypic resistance varied among the isolates from different sources, and in many cases, the presence of resistance genes was not consistent with the resistance to the corresponding antimicrobials. These results are very significant for veterinary practice and public health.
全球范围内，沙门氏菌菌株的抗菌耐药性正在迅速增加，这对动物和公共卫生构成了重大威胁。在本研究中，对2008年至2015年期间在中国收集的249株猪源沙门氏菌进行了检测，其中包括155株来自患病猪的临床分离株和94株来自健康猪的非临床分离株。根据七种抗菌药物的最低抑菌浓度，96.4%的分离株对至少一种测试抗生素耐药，81.0%的分离株表现出多重耐药性。四环素的抗菌耐药率最高（85.9%），头孢噻肟的最低（13.3%）。来自患病猪的分离株的抗菌耐药水平显著高于来自健康猪的分离株。来自健康猪的22株分离株对环丙沙星耐药，这可能会抑制氟喹诺酮类药物对由受污染食物引起的人类细菌性食源性中毒和感染的治疗效果。此外，与2008年至2010年期间分离的菌株相比，2013年至2015年期间从患病猪分离的菌株对头孢噻肟的耐药性显著更高。进一步研究表明，不同来源分离株的表型和基因型耐药性之间的相关性各不相同，在许多情况下，耐药基因的存在与对相应抗菌药物的耐药性并不一致。这些结果对兽医实践和公共卫生具有重要意义。
