Department of Conservative Dentistry, Institute of Biomedical Sciences, Tokushima University Graduate School, 3-18-15 Kuramoto-cho, Tokushima, 770-8504, Japan.
Department of Oral Health Care Promotion, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, Japan.
Inflammation. 2019 Aug;42(4):1456-1462. doi: 10.1007/s10753-019-01007-z.
Sudachitin, a polymethoxylated flavonoid found in the skin of Citrus sudachi, is a biologically active substance. The aim of this study was to examine whether sudachitin could be used to inhibit the expression of matrix metalloproteinase (MMP)-1 and MMP-3, which are involved in the destruction of periodontal tissues in periodontal lesions, in tumor necrosis factor (TNF)-α-stimulated human periodontal ligament cells (HPDLC). Sudachitin suppressed TNF-α-induced MMP-1 and MMP-3 production in HPDLC. On the other hand, it enhanced tissue inhibitor of metalloproteinase (TIMP)-1 expression. The level of Akt phosphorylation in the TNF-α-stimulated HPDLC was decreased by sudachitin treatment. Moreover, an Akt inhibitor reduced MMP-1 and MMP-3 production and increased TIMP-1 production. These findings indicate that sudachitin reduces MMP-1 and MMP-3 production in TNF-α-stimulated HPDLC by inhibiting the Akt pathway.
柚皮素是一种存在于柑橘属柚子果皮中的多甲氧基类黄酮，是一种具有生物活性的物质。本研究旨在探讨柚皮素是否可用于抑制肿瘤坏死因子（TNF）-α刺激下人牙周韧带细胞（HPDLC）中参与牙周组织破坏的基质金属蛋白酶（MMP）-1和 MMP-3的表达。柚皮素可抑制 TNF-α诱导的 HPDLC 中 MMP-1 和 MMP-3 的产生。另一方面，它可增强金属蛋白酶组织抑制剂（TIMP）-1 的表达。柚皮素处理可降低 TNF-α刺激的 HPDLC 中 Akt 的磷酸化水平。此外，Akt 抑制剂可减少 MMP-1 和 MMP-3 的产生，增加 TIMP-1 的产生。这些结果表明，柚皮素通过抑制 Akt 通路减少 TNF-α刺激的 HPDLC 中 MMP-1 和 MMP-3 的产生。
