Rabin E Z, Weinberger V, Tattrie B
Clin Chim Acta. 1977 Jul 15;78(2):235-42. doi: 10.1016/0009-8981(77)90311-4.
Poly(C)-avid ribonucleases of molecular weight 33 000 are present in the serum, cerebrospinal fluid and urine of humans. Purified human urinary ribonuclease was used to produce a monospecific antibody in rabbits. The antibody was capable of: (i) inhibiting the enzyme activities in the serum, CSF, and urine; (ii) reacting with antigens in the serum and CSF. The antigens in the serum, CSF and urine were found to be immunologically identical. Immunoelectrophoresis data suggested that the urinary and CSF RNAase are chemically identical. Succesful renal transplantation reduced elevated serum RNAase to normal levels. The data suggest that the most likely source of both urinary and CSF ribonuclease activity is the blood stream.
分子量为33000的聚（C）-亲和核糖核酸酶存在于人类的血清、脑脊液和尿液中。用纯化的人尿核糖核酸酶在兔体内制备了单特异性抗体。该抗体能够：（i）抑制血清、脑脊液和尿液中的酶活性；（ii）与血清和脑脊液中的抗原发生反应。发现血清、脑脊液和尿液中的抗原在免疫学上是相同的。免疫电泳数据表明，尿和脑脊液中的核糖核酸酶在化学上是相同的。成功的肾移植使升高的血清核糖核酸酶降至正常水平。数据表明，尿和脑脊液核糖核酸酶活性最可能的来源是血流。
