Liu Chi-Te, Lin Shih-Yao, Hameed Asif, Liu You-Cheng, Hsu Yi-Han, Wong Wai-Tak, Tseng Ching-Han, Lur Huu-Sheng, Young Chiu-Chung
Agricultural Biotechnology Research Center, Academia Sinica, Taipei 115, Taiwan, ROC.
Institute of Biotechnology, National Taiwan University, Taiwan, ROC.
Int J Syst Evol Microbiol. 2017 Feb;67(2):183-189. doi: 10.1099/ijsem.0.001565. Epub 2017 Feb 24.
A polyphasic approach was used to characterize a novel bacterium, designated strain TPP412T, isolated from a paddy soil in Taiwan. Strain TPP412T was Gram-stain-negative, facultatively anaerobic, rod-shaped, motile with a single polar flagellum and lacked bacteriochlorophyll. Growth was observed at 24-45 °C (optimal 25 °C), at pH 5.0-10.0 (optimal pH 7.0) and with 0-0.75 % (w/v) NaCl. Strain TPP412T showed highest 16S rRNA gene sequence similarity to members of the genera Rhodocyclus (94.1-94.5 %), Azospira (93.9-94.5 %) and Propionivibrio (93.4-94.4 %) and established a discrete taxonomic lineage in phylogenetic analysis. The major fatty acids found in strain TPP412T were C12 : 0, C12 : 0 3-OH, iso-C15 : 0 3-OH, C16 : 0, C16 : 1ω7c/C16 : 1ω6c and C18 : 1ω7c/C18 : 1ω6c. The major polar lipids consisted of phosphatidylmonomethylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and an unidentified lipid. The polyamine pattern showed a predominance of putrescine and a minor amount of spermidine. The DNA G+C content was 58.4 mol% and the predominant quinone system was ubiquinone-8 (Q-8). The low 16S rRNA gene sequence similarity values (≤94.5%) and distinct phylogenetic clustering clearly distinguished strain TPP412T from other representatives of the family Rhodocyclaceae. Based on the discrete phylogenetic, phenotypic and chemotaxonomic traits together with results of comparative 16S rRNA gene sequence analysis, strain TPP412T is considered to represent a novel species of a new genus in the family Rhodocyclaceae, for which the name Oryzomicrobium terrae gen. nov., sp. nov. is proposed. The type strain of Oryzomicrobium terrae is TPP412T (=BCRC 80905T=JCM 30814T).
采用多相分类法对从台湾稻田土壤中分离出的一株新细菌TPP412T进行特征描述。菌株TPP412T革兰氏染色阴性，兼性厌氧，杆状，具单根极生鞭毛，能运动，且缺乏细菌叶绿素。在24 - 45 °C（最适温度25 °C）、pH 5.0 - 10.0（最适pH 7.0）和0 - 0.75 %（w/v）NaCl条件下可观察到生长。菌株TPP412T的16S rRNA基因序列与红环菌属（94.1 - 94.5 %）、偶氮螺菌属（93.9 - 94.5 %）和丙酸弧菌属（93.4 - 94.4 %）成员的序列相似性最高，并在系统发育分析中形成一个独立的分类谱系。菌株TPP412T中发现的主要脂肪酸为C12 : 0、C12 : 0 3 - OH、异C15 : 0 3 - OH、C16 : 0、C16 : 1ω7c/C16 : 1ω6c和C18 : 1ω7c/C18 : 1ω6c。主要极性脂类包括磷脂酰单甲基乙醇胺、双磷脂酰甘油、磷脂酰甘油和一种未鉴定的脂类。多胺模式显示腐胺占优势，亚精胺含量较少。DNA G + C含量为58.4 mol%，主要醌系统为泛醌 - 8（Q - 8）。16S rRNA基因序列相似性值较低（≤94.5%）以及明显的系统发育聚类清楚地将菌株TPP412T与红环菌科的其他代表菌株区分开来。基于独立的系统发育、表型和化学分类学特征以及比较16S rRNA基因序列分析结果，菌株TPP412T被认为代表红环菌科一个新属的一个新物种，为此提出新属名为稻田微菌属（Oryzomicrobium），新种名为稻田稻田微菌（Oryzomicrobium terrae）。稻田稻田微菌的模式菌株为TPP412T（=BCRC 80905T=JCM 30814T）。
