College of Oceanology and Food Science, Quanzhou Normal University, Quanzhou, China.
Fujian Province Key Laboratory for the Development of Bioactive Material From Marine Algae, Quanzhou, China.
Antonie Van Leeuwenhoek. 2021 Oct;114(10):1551-1563. doi: 10.1007/s10482-021-01621-8. Epub 2021 Jul 22.
A novel bright-yellow pigmented bacterial strain SM2-F was isolated from a mangrove sediment collected at the mangrove coast of Luoyang estuary, Quanzhou, China. Strain SM2-F was Gram-stain-negative, catalase-weak positive, oxidase-positive, rod-shaped, non-flagellated and non-motile. Growth of strain SM2-F was observed at 20-40 °C (optimum, 30 °C), pH 6.0-8.0 (optimum, pH 7.0) and in the presence of 1.0-4.0% NaCl (optimum, 2.0% NaCl). Flexirubin-pigment was absent, and carotenoid-pigment was present. Phylogenetic analysis of 16S rRNA gene sequence placed strain SM2-F into the family Flavobacteriaceae and shared the maximum sequence similarity with Aequorivita soesokkakensis RSSK-12 of 92.5%. Whole genomic comparison between strain SM2-F and close relatives suggested a novel species of a novel genus. The predominant quinone of strain SM2-F was menaquinone (MK)-6. The major fatty acids (> 10%) comprised iso-C G (32.4%) and iso-C (29.1%). The polar lipid profile consisted of phosphatidylethanolamine, two unidentified aminolipids and four unidentified lipids. The complete genome size was 4,094,245 bp with DNA G + C content of 36.0 mol%. Based on the data of polyphasic study, strain SM2-F was considered to represent a novel species of a novel genus, for which the name Aegicerativicinus sediminis gen. nov., sp. nov., was proposed. The type strain was SM2-F (= MCCC 1K04383 = KCTC 82361).
一株新型亮黄色着色细菌菌株 SM2-F 从中国泉州洛阳河口红树林海岸采集的红树林沉积物中分离得到。菌株 SM2-F 革兰氏阴性，过氧化氢酶弱阳性，氧化酶阳性，杆状，无鞭毛，不运动。菌株 SM2-F 的生长温度为 20-40°C（最佳温度为 30°C），pH 值为 6.0-8.0（最佳 pH 值为 7.0），并在 1.0-4.0%NaCl（最佳 2.0%NaCl）存在的情况下生长。不存在 flexirubin 色素，存在类胡萝卜素色素。16S rRNA 基因序列的系统发育分析将菌株 SM2-F 归入黄杆菌科，并与 Aequorivita soesokkakensis RSSK-12 的序列相似度最高，为 92.5%。菌株 SM2-F 与近亲之间的全基因组比较表明，这是一个新属的新种。菌株 SM2-F 的主要醌为menaquinone (MK)-6。主要脂肪酸（>10%）包括 iso-C G（32.4%）和 iso-C（29.1%）。极性脂谱由磷脂酰乙醇胺、两种未鉴定的胺脂和四种未鉴定的脂组成。完整基因组大小为 4094245bp，DNA G+C 含量为 36.0mol%。基于多相研究的数据，菌株 SM2-F 被认为代表一个新属的新种，因此建议将其命名为 Aegicerativicinus sediminis gen. nov.，sp. nov.。模式菌株为 SM2-F（=MCCC 1K04383=KCTC 82361）。
