Key Laboratory of Natural Products Chemical Biology, Ministry of Education, School of Pharmaceutical Sciences, Shandong University, 250012 Jinan, PR China.
Key Laboratory of Natural Products Chemical Biology, Ministry of Education, School of Pharmaceutical Sciences, Shandong University, 250012 Jinan, PR China.
Biosens Bioelectron. 2014 Sep 15;59:64-74. doi: 10.1016/j.bios.2014.03.014. Epub 2014 Mar 15.
Aptasensors are aptamer-based biosensors with excellent recognition capability towards a wide range of targets. Specially, there have been ever-growing interests in the development of aptasensors for the detection of small molecules. This phenomenon is contributed to two reasons. On one hand, small biomolecules play an important role in living organisms with many kinds of biological function, such as antiarrhythmic effect and vasodilator activity of adenosine. On the other hand, the concentration of small molecules can be an indicator for disease diagnosis, for example, the concentration of ATP is closely associated with cell injury and cell viability. As a potential analysis tool in the construction of aptasensors, optical analysis has attracted much more interest of researchers due to its high sensitivity, quick response and simple operation. Besides, it promises the promotion of aptasensors in performance toward a new level. Review the development of optical aptasensors for small biomolecules will give readers an overall understanding of its progress and provide some theoretical guidelines for its future development. Hence, we give a mini-review on the advance of optical aptasensors for small biomolecules. This review focuses on recent achievements in the design of various optical aptasensors for small biomolecules, containing fluorescence aptasensors, colorimetric aptasensors, chemiluminescence aptasensors and other optical aptasensors.
适配体传感器是基于适配体的生物传感器，对广泛的目标具有出色的识别能力。特别是，人们对开发用于检测小分子的适配体传感器越来越感兴趣。这种现象有两个原因。一方面，小分子在生物体中发挥着重要作用，具有多种生物学功能，如腺苷的抗心律失常作用和血管扩张活性。另一方面，小分子的浓度可以作为疾病诊断的指标，例如，ATP 的浓度与细胞损伤和细胞活力密切相关。作为适配体传感器构建中的一种潜在分析工具，由于其高灵敏度、快速响应和简单操作，光学分析引起了研究人员更多的兴趣。此外，它有望将适配体传感器的性能提升到一个新的水平。综述小分子的光学适配体传感器的发展将使读者全面了解其进展，并为其未来发展提供一些理论指导。因此，我们对小分子的光学适配体传感器的进展进行了简要综述。本综述重点介绍了用于小分子的各种光学适配体传感器的设计的最新进展，包括荧光适配体传感器、比色适配体传感器、化学发光适配体传感器和其他光学适配体传感器。
