Hernández Ana L, Casquel Rafael, Holgado Miguel, Cornago Iñaki, Fernández Fátima, Ciaurriz Paula, Sanza Francisco J, Santamaría Beatriz, Maigler María V, Laguna María Fe
Opt Lett. 2016 Dec 1;41(23):5430-5433. doi: 10.1364/OL.41.005430.
In our previous work we demonstrated for the first time, to the best of our knowledge, the experimental capability of resonant nanopillars (R-NP) arrays as biochemical transducers. In this Letter, we provide evidence of the capability and suitability of R-NP arrays on a chip to function as label-free optical multiplexed biosensors. R-NP are based on SiN/SiO Bragg reflectors with a cavity of SiO. In order to demonstrate the biosensing performance, R-NP were biofunctionalized by the immobilization of IgG antibodies acting as a bioreceptor. This immobilization was carried out through the silanization of the pillars sensing surface with APTMS (3-aminopropyltrimethoxysilane). R-NP were integrated in eight different sensing arrays on a quartz surface chip. An optical fiber bundle monitored each sensing array vertically and independently after each biofunctionalization step, and subsequently after every recognition event of increasing concentrations of anti-IgGs. The results report a novel multiplexed optical biosensor made of eight sensing arrays on a chip with promising performance and yield.
据我们所知，在我们之前的工作中，我们首次展示了共振纳米柱（R-NP）阵列作为生化传感器的实验能力。在本信函中，我们提供了芯片上的R-NP阵列作为无标记光学多路复用生物传感器的功能和适用性的证据。R-NP基于具有SiO腔的SiN/SiO布拉格反射器。为了展示生物传感性能，通过固定作为生物受体的IgG抗体对R-NP进行生物功能化。这种固定是通过用APTMS（3-氨丙基三甲氧基硅烷）对柱传感表面进行硅烷化来实现的。R-NP被集成在石英表面芯片上的八个不同传感阵列中。在每个生物功能化步骤之后，以及随后在抗IgG浓度不断增加的每次识别事件之后，用光纤束垂直且独立地监测每个传感阵列。结果报告了一种由芯片上的八个传感阵列制成的新型多路复用光学生物传感器，具有良好的性能和产量。
