昼夜地氟烷麻醉对大鼠褪黑素水平的影响。
Effect of Day and Night Desflurane Anaesthesia on Melatonin Levels in Rats.
作者信息
Özer Figen Datlı, Öçmen Elvan, Akan Pınar, Erdost Hale Aksu, Korkut Sezen, Gökmen Ali Necati
机构信息
Department of Anaesthesiology and Reanimation, Dokuz Eylül University School of Medicine, İzmir, Turkey.
Department of Biochemistry, Dokuz Eylül University School of Medicine, İzmir, Turkey.
出版信息
Turk J Anaesthesiol Reanim. 2016 Aug;44(4):190-194. doi: 10.5152/TJAR.2016.88609. Epub 2016 Aug 1.
OBJECTIVE
The aim of this study is to investigate the effect of day and night administration of desflurane anaesthesia on melatonin levels in rats.
METHODS
Twenty-four 15-day-old rats were included in the study and were divided into four groups. The rats were anaesthetised between 19:00-01:00 (night group) and 07:00-13:00 (day group) with 5.7% desflurane concentration in 6 L min 100% oxygen. 6 L min oxygen was administered to the control groups. At the end of 6 h of anaesthesia, blood samples were taken, and rats were sacrificed. Blood samples were centrifuged and melatonin levels from plasma samples were measured with radioimmunoassay.
RESULTS
There was a statistically significant difference between the groups (p=0.007). Between group day control and group night control there was a statistically significant difference (p=0.042). Further, there was a significant difference between group day control and night desfluran as well (p=0.024). We could not find any difference between other groups.
CONCLUSION
This study showed that 6 hours of 5.7% desflurane anaesthesia during day and night hours did not significantly change melatonin levels.
目的
本研究旨在探讨地氟烷麻醉的昼夜给药对大鼠褪黑素水平的影响。
方法
本研究纳入24只15日龄大鼠，分为四组。在19:00 - 01:00（夜间组）和07:00 - 13:00（白天组）期间，用5.7%地氟烷浓度在6 L/min 100%氧气中对大鼠进行麻醉。对照组给予6 L/min氧气。麻醉6小时结束时，采集血样并处死大鼠。血样离心后，用放射免疫分析法测定血浆样本中的褪黑素水平。
结果
各组之间存在统计学显著差异（p = 0.007）。白天对照组和夜间对照组之间存在统计学显著差异（p = 0.042）。此外，白天对照组和夜间地氟烷组之间也存在显著差异（p = 0.024）。我们在其他组之间未发现任何差异。
结论
本研究表明，昼夜6小时的5.7%地氟烷麻醉并未显著改变褪黑素水平。
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