Department of Cardiovascular and Metabolic Sciences, Lerner Research Institute, Cleveland Clinic Lerner College of Medicine, Cleveland Clinic and Department of Molecular Medicine, Case Western Reserve University, Cleveland, OH 44106, USA.
Department of Molecular and Cell Biology, University of California, Merced, Merced, CA 95343, USA.
Nucleic Acids Res. 2020 Jul 27;48(13):7066-7078. doi: 10.1093/nar/gkaa464.
During nuclear maturation of most eukaryotic pre-messenger RNAs and long non-coding RNAs, introns are removed through the process of RNA splicing. Different classes of introns are excised by the U2-type or the U12-type spliceosomes, large complexes of small nuclear ribonucleoprotein particles and associated proteins. We created intronIC, a program for assigning intron class to all introns in a given genome, and used it on 24 eukaryotic genomes to create the Intron Annotation and Orthology Database (IAOD). We then used the data in the IAOD to revisit several hypotheses concerning the evolution of the two classes of spliceosomal introns, finding support for the class conversion model explaining the low abundance of U12-type introns in modern genomes.
在大多数真核前信使 RNA 和长非编码 RNA 的核成熟过程中，通过 RNA 剪接去除内含子。不同类别的内含子由 U2 型或 U12 型剪接体切除，剪接体是由小核核糖核蛋白颗粒和相关蛋白组成的大型复合物。我们创建了 intronIC，这是一个用于为给定基因组中的所有内含子分配内含子类别的程序，并在 24 个真核基因组上使用它创建了内含子注释和同源数据库 (IAOD)。然后，我们使用 IAOD 中的数据重新审视了几个关于两种剪接体内含子进化的假说，为解释现代基因组中 U12 型内含子低丰度的类转换模型提供了支持。
