Li Yanxia, Chen Yiting, Huang Lu, Lou BenYong, Chen Guonan
Department of Chemistry and Chemical Engineering, Minjiang University, Fuzhou, Fujian 350108, China.
Ministry of Education Key Laboratory of Analysis and Detection Technology for Food Safety (Fuzhou University), Fuzhou, 350002, China.
Analyst. 2017 Jan 16;142(2):302-309. doi: 10.1039/c6an02121b.
A kind of protein imprinted over magnetic FeO@Au multifunctional nanoparticles (NPs) with multiple binding sites was synthesized and investigated. Magnetic FeO@Au NPs as carrier materials were modified with 4-mercaptophenylboronic acid (MPBA) and mercaptopropionic acid (MPA) to introduce boronic acids and carboxyl groups. Using Bovine Hemoglobin (BHb) as a template, a polydopamine(PDA)-based molecular imprinted film was fabricated to produce a kind of magnetic molecularly imprinted nanoparticle (MMIP), possessing multiple binding sites with benzene-diol, amino groups, boronic acids and carboxyl groups. The MMIPs exhibited an excellent imprinting effect and adsorption capacity (89.65± 0.38 mg g) toward the template protein. The results show that the MMIPs reached saturated adsorption at 0.5 mg mL within 90 min. The synthesized MMIPs are suitable for the removal and enrichment of the template protein in proteomics. The strategy of multiple binding sites paves the way for the preparation of functional nanomaterials in molecular imprinting techniques.
合成并研究了一种印迹在具有多个结合位点的磁性FeO@Au多功能纳米粒子（NPs）上的蛋白质。以磁性FeO@Au NPs作为载体材料，用4-巯基苯硼酸（MPBA）和巯基丙酸（MPA）进行修饰，以引入硼酸基团和羧基。以牛血红蛋白（BHb）为模板，制备了一种基于聚多巴胺（PDA）的分子印迹膜，以制备一种对苯二酚、氨基、硼酸基团和羧基具有多个结合位点的磁性分子印迹纳米粒子（MMIP）。MMIPs对模板蛋白表现出优异的印迹效果和吸附容量（89.65±0.38 mg g）。结果表明，MMIPs在90分钟内于0.5 mg mL达到饱和吸附。合成的MMIPs适用于蛋白质组学中模板蛋白的去除和富集。多结合位点策略为分子印迹技术中功能纳米材料的制备铺平了道路。
