Li Yanxia, Huang Lu, Wang Xiuping, Chen Yiting
Department of Chemistry and Chemical Engineering, Minjiang University.
Anal Sci. 2017;33(10):1105-1110. doi: 10.2116/analsci.33.1105.
A study of an electrochemical sensor based on bovine hemoglobin (BHb) imprinted magnetic nanoparticles (MIPs) was investigated. First, BHb MIPs were successfully synthesized with magnetic FeO@Au nanoparticles as carrier by surface modification of mercapto propionic acid for introducing carboxyl groups, combined with dopamine as the functional monomer and BHb as the template protein. Then, the MIPs were modified to the surface of a glassy carbon electrode (GCE) for electrochemical analysis by using cyclic voltammetry in the potassium ferricyanide solution. Results show that there was a good dynamic response between electrochemical signals and the adsorption amount of protein in the range of 0.05 to 0.5 mg mL. Results show that the sensor exhibits a significant specific recognition toward the template protein via selective test and can be used for analysis of serum samples. The synthesized MIPs are suitable for the removal and enrichment of template protein in proteomics. At the same time, the proposed electrochemical sensor can be used for recognition of BHb.
对基于牛血红蛋白（BHb）印迹磁性纳米粒子（MIPs）的电化学传感器进行了研究。首先，以磁性FeO@Au纳米粒子为载体，通过巯基丙酸表面改性引入羧基，结合多巴胺作为功能单体、BHb作为模板蛋白，成功合成了BHb MIPs。然后，在铁氰化钾溶液中利用循环伏安法将MIPs修饰到玻碳电极（GCE）表面进行电化学分析。结果表明，在0.05至0.5 mg/mL范围内，电化学信号与蛋白质吸附量之间存在良好的动态响应。结果表明，该传感器通过选择性测试对模板蛋白表现出显著的特异性识别，可用于血清样本分析。合成的MIPs适用于蛋白质组学中模板蛋白的去除和富集。同时，所提出的电化学传感器可用于识别BHb。
