Posa Francesca, Di Benedetto Adriana, Colaianni Graziana, Cavalcanti-Adam Elisabetta A, Brunetti Giacomina, Porro Chiara, Trotta Teresa, Grano Maria, Mori Giorgio
Department of Clinical and Experimental Medicine, Medical School, University of Foggia, Foggia, Italy.
Section of Human Anatomy and Histology, Department of Basic and Medical Sciences, Neurosciences and Sense Organs, University of Bari, Bari, Italy.
Stem Cells Int. 2016;2016:9150819. doi: 10.1155/2016/9150819. Epub 2016 Nov 13.
1,25-Dihydroxyvitamin D (1,25(OH)D), the active metabolite of vitamin D (Vit D), increases intestinal absorption of calcium and phosphate, maintaining a correct balance of bone remodeling. Vit D has an anabolic effect on the skeletal system and is key in promoting osteoblastic differentiation of human Mesenchymal Stem Cells (hMSCs) from bone marrow. MSCs can be also isolated from the immature form of the tooth, the dental bud: Dental Bud Stem Cells (DBSCs) are adult stem cells that can effectively undergo osteoblastic differentiation. In this work we investigated the effect of Vit D on DBSCs differentiation into osteoblasts. Our data demonstrate that DBSCs, cultured in an opportune osteogenic medium, differentiate into osteoblast-like cells; Vit D treatment stimulates their osteoblastic features, increasing the expression of typical markers of osteoblastogenesis like RUNX2 and Collagen I (Coll I) and, in a more important way, determining a higher production of mineralized matrix nodules.
1,25-二羟基维生素D（1,25(OH)D）是维生素D（Vit D）的活性代谢产物，可增加肠道对钙和磷的吸收，维持骨重塑的正确平衡。Vit D对骨骼系统具有合成代谢作用，是促进人骨髓间充质干细胞（hMSCs）向成骨细胞分化的关键因素。间充质干细胞也可从未成熟的牙齿——牙胚中分离得到：牙胚干细胞（DBSCs）是能够有效进行成骨细胞分化的成体干细胞。在这项研究中，我们调查了Vit D对DBSCs向成骨细胞分化的影响。我们的数据表明，在合适的成骨培养基中培养的DBSCs可分化为成骨样细胞；Vit D处理可刺激其成骨细胞特征，增加成骨细胞生成的典型标志物如RUNX2和I型胶原蛋白（Coll I）的表达，更重要的是，可促使矿化基质结节产生量增加。
