Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China.
Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China.
Arch Virol. 2021 Apr;166(4):1259-1262. doi: 10.1007/s00705-021-04990-7. Epub 2021 Feb 13.
In 2019, diarrhea cases occurred on cattle farms in Qionglai and Guang'an, Sichuan Province. Two out of 20 (10%) serum and nasal swab samples were positive when tested using a bovine viral diarrhea virus (BVDV) antigen-capture ELISA kit. Two non-cytopathic strains of BVDV were isolated and named QL1903 and GA190608, respectively. The nucleotide sequences of the genomes of the two isolates were 89.52% identical. Phylogenetic analysis based on the 5'-UTR sequence revealed that the BVDV isolate QL1903 belonged to BVDV subtype 1b, whereas isolate GA190608 clustered with strains HN1814, EN-19, and BJ09_26 in a separate branch, which has tentatively been classified as a new genetic subtype, "1v".
2019 年，四川省邛崃市和广安市的奶牛场发生腹泻病例。使用牛病毒性腹泻病毒（BVDV）抗原捕获 ELISA 试剂盒检测时，20 份血清和鼻拭子样本中有 2 份（10%）呈阳性。分离到两株非致细胞病变的 BVDV，分别命名为 QL1903 和 GA190608。两株分离株基因组的核苷酸序列同源性为 89.52%。基于 5'-UTR 序列的系统进化分析表明，BVDV 分离株 QL1903 属于 BVDV 亚型 1b，而分离株 GA190608 与 HN1814、EN-19 和 BJ09_26 等菌株在一个单独的分支聚类，该分支暂被归类为一个新的遗传亚型“1v”。
